Wnt/β-catenin基因对骨髓瘤患者间充质干细胞成骨潜能的影响

黄瑞宏; 王宁; 李祎; 张琼丽; 楼瑾; 李明; 杜新; 卓家才

doi:10.13201/j.issn.1004-2806.2012.02.009

Wnt/β-catenin基因对骨髓瘤患者间充质干细胞成骨潜能的影响

- 广东省深圳市血液病研究所深圳市第二人民医院血液科(广东深圳, 518035)
基金项目:
深圳市科技信息局科研基金资助项目(No:201002034)

详细信息

通讯作者: 杜新,E-mail:duxingz@medmail.com.cn

中图分类号: R331.2

收稿日期: 2011-08-05

Effect of Wnt/β-catenin on osteogenic potential of mesenchymal stem cells from myeloma patients

- 1. Shenzhen Institute of Hematology, Shenzhen, 518035, China

More Information

Corresponding author: DU Xin, E-mail: duxingz@medmail.com.cn

Received Date: 05 August 2011

摘要

摘要: 目的:探讨Wnt/β-catenin基因和多发性骨髓瘤骨病的关系。方法:分离培养多发性骨髓瘤患者和正常人的骨髓间充质干细胞(MSCs),体外诱导分化成骨细胞,茜素红实验检测矿化物沉积、荧光定量RT-PCR方法检成骨指标(OPN、OC、ALP、Cbfal) 和Wnt/β-catenin mRNA表达,分析MSCs细胞成骨能力变化及两组间Wnt/β-catenin mRNA表达差异。结果:骨髓MSCs体外成骨诱导后,茜素红染色阳性,呈现明显红色钙化结节;MSCs体外诱导成骨细胞,试验组与对照组比较,成骨指标OPN、OC、ALP、 Cbfαl mRNA表达降低(P＜0.05);骨髓MSCs体外成骨诱导后β-catenin mRNA表达降低(P＜0.05)。结论:骨髓MSCs体外可成功诱导分化为成骨细胞;多发性骨髓瘤患者MSCs向成骨细胞分化潜能比正常人降低,Wnt/β-catenin 可作为多发性骨髓瘤骨病潜在治疗靶点。
- 骨髓间充质干细胞 /
- 成骨细胞 /
- 实时聚合酶链反应 /
- Wnt/β-catenin
Abstract: Objective:To investigate the relationship between Wnt/β-catenin and multiple myeloma bone disease (MBD). Method:Bone marrow mononuclear cells from MM and controls were isolated,cultured,expanded and then induced to osteogenic differentiation. Realtime Quantitative PCR was employed to detect the osteogenic markers,including Wnt/β-catenin,OPN,OC,ALP,Cbfαl.Alizarin red staining was conducted for detecting mineral deposition. The mRNA expressions of Wnt/β-catenin and osteogenic potential in the two groups were analyzed. Result:Positive alizarin red staining and the red calcium nodules appeared on the MSC post-osteogenic induction in vitro. The mRNA expressions of OPN,OC,ALP,and Cbfαl were significantly lower than those of control groups(P＜0.05). The mRNA expression of β-catenin on the MSC post-osteogenic induction in vitro in the experimental groups was significantly lower than those of control groups(P＜0.05). Conclusion:MSCs can be successfully induced to osteoblasts. The osteogenic potential of MSCs in MM patients is lower than controls. Wnt/β-catenin may present a novel target for the future therapy of MBD.
- bone marrow mesenchymal stem cells /
- osteoblasts /
- real-time polymerase chain reaction /
- Wnt/β-catenin

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