CD123在成熟B淋巴细胞肿瘤中的表达及意义

杜雯; 郑金娥; 马耀坤; 李娟; 刘伟; 姚军霞; 贺艳丽

doi:10.13201/j.issn.1004-2806.2017.07.008

CD123在成熟B淋巴细胞肿瘤中的表达及意义

- 1. 华中科技大学附属协和医院干细胞中心(武汉, 430022);
- 2. 武汉康圣达医学检验所有限公司

详细信息

通讯作者: 贺艳丽,E-mail:heyanlicn@hust.edu.cn

中图分类号: R733.4

收稿日期: 2016-12-07

The expression and significance of CD123 in mature B-cell neoplasm

- 1. Stem Cell Center, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China;
- 2. Kindstar Medical Laboratory Co Ltd in Wuhan

More Information

Corresponding author: HE Yanli, E-mail: heyanlicn@hust.edu.cn

Received Date: 07 December 2016

摘要

摘要: 目的:探讨CD123在不同类型成熟B淋巴细胞肿瘤诊断和鉴别诊断中的意义。方法:选取297例成熟B淋巴细胞肿瘤患者作为研究对象,疾病类型包括慢性淋巴细胞白血病/小淋巴细胞淋巴瘤(CLL/SLL),套细胞淋巴瘤(MCL),滤泡性淋巴瘤(FL),脾边缘区淋巴瘤(SMZL),毛细胞白血病(HCL)和变异型HCL(HCLv)。采集患者的骨髓标本,通过流式细胞术分析其免疫表型,以及各类型疾病中异常淋巴细胞群CD123表达的抗原阳性比例及强度。结果:不同类型成熟B淋巴细胞肿瘤患者骨髓异常淋巴细胞群中,其CD123表达率及表达强度存在较大差异。其中156例CLL/SLL中CD123阳性比例约占3.21%,表达强度较低;45例MCL中CD123阳性比例约占8.89%,表达强度较高;62例FL中CD123阳性比例约占4.84%,表达强度较低;20例SMZL CD123阳性比例约占15.00%,表达强度较高;10例HCL CD123阳性比例占100%,且其表达强度最高;但4例HCLv未见CD123阳性表达。结论:CD123在成熟B淋巴细胞肿瘤常见类型中广泛表达,其表达率和强度尤其可作为HCL与其他亚类进行鉴别的重要指标之一。
- 成熟B淋巴细胞肿瘤 /
- CD123 /
- 流式细胞术
Abstract: Objective:To explore the significance of CD123 in the diagnosis and differential diagnosis in different types of mature B-cell neoplasms.Method:Bone marrow cells were collected from 297 patients with mature B-cell neoplasms,including CLL/SLL,MCL,FL,SMZL and HCL,HCLv.By using flow cytometry,the immunophenotype,CD123 antigen expression and fluorescence intensity in the bone marrow malignant cells were analyzed.Result:There were significant differences in CD123 expression and fluorescence intensity in the malignant cells of CLL/SLL,MCL,FL,SMZL,HCL and HCLv.CD123 was dim positive in 3.21% of 156 CLL/SLL,4.84% of 62 FL and was positive in 8.89% of 45 MCL,15.00% of 20 SMZL,especially CD123 was negative in all 4 HCLv and bright positive in all 10 HCL.Conclusion:CD123 is widely expressed in mature B-cell neoplasm and bright CD123 is useful in differentiate HCL from other types of mature B-cell neoplasm.
- mature B-cell neoplasm /
- CD123 /
- flow cytometry

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