Analysis of platelet activation and function in three types of blood cell separator
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摘要: 目的:分析3种机型血细胞分离机Amicus、Trima和Mcs+采集血小板活化和功能的差异。方法:选择2019年单采血小板的献血者60例,按照3种机型所有献血者被随机分成3组,每组各20例。检测各组采集血小板活化指标PAC-1、CD62P和用血栓弹力图评估功能,进行统计分析。结果:3种机型采集血小板活化指标CD62P均高于正常值,Amicus采集血小板活化指标CD62P和PAC-1较另2种血细胞分离机比较差异有统计学意义(P＜0.05)。3种机型血小板功能指标和血小板计数比较差异无统计学意义(P>0.05)。结论:3种机型血细胞分离机采集的血小板,均存在一定程度血小板活化;不同分离方式所采集的单采血小板其活化程度之间存在差异。Abstract: Objective:To analyze the differences of platelet activation and function among Amicus,TrimA and MCS +blood cell separator.Methods:60 cases of platelet donors in 2019 were selected.They were randomly divided into 3 groups according to the three machine models,with 20 cases in each group.The platelet activation indexes PAC-1 and CD62 p were detected in each group.In addition,thromboelastography was used to evaluate platelet function.These data were statistical analyzed.Results:The platelet activation index CD62 P collected by three models was higher than the normal value.The platelet activation indexes CD62 P and PAC-1 collected by Amicus were significantly different from the other two blood cell separators(P＜0.05).There was no significant difference in platelet function index and platelet count among the three groups(P>0.05).Conclusion:Platelets collected by three types of blood cell separators are activated to a certain extent.There are differences in the activation levels of apheresis platelets collected by different separation methods.
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