Abstract: Objective: To provide the basis for the change of HCV antibody detection reagent from indirect method to double antigen sandwich method in our blood center.Methods: Samples of unpaid blood donors in our center from 2018 to 2020 were randomly selected to detect anti HCV antibody by enzyme-linked immunosorbent assay(ELISA) indirect double reagent(a, b) and double antigen sandwich double reagent(A1, B1), and HCV RNA and nucleic acid were detected at the same time; the detection results of four kinds of anti HCV ELISA kits and the positive coincidence rate of single reagent positive samples were statistically analyzed.Results: A total of 116 844 and 98 903 blood donors were detected by the double reagents of anti HCV ELISA indirect method and double antigen sandwich method. The positive rate of anti HCV antibody single reagent of indirect method was 0.088%(102/116 843) and that of anti HCV antibody single reagent of double antigen sandwich method was 0.012%(12/98 903), the difference was statistically significant(P<0.05). The positive coincidence rates of A, B, A1 and B1 were A(61.90%)>B(54.95)%>A1(50.00%) and B1(50.00%), respectively. There was no statistical difference between the four reagents(P>0.05). The nucleic acid RNA of single reagent positive samples was negative.Conclusion: Tere are differences in gender and age distribution of anti HCV antibody double reagent positive blood donors in this region, which has guiding significance for future recruitment of low-risk blood donors; anti HCV double antigen sandwich ELISA has higher specificity, reduces false positive rate, and ensures the safety of clinical blood use.