Analysis and research on unqualified blood HBsAg, anti-HCV, anti-HIV and anti-TP of unpaid blood donors
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摘要: 目的:分析献血者乙型肝炎表面抗原(HBsAg)、丙型肝炎病毒抗体(抗-HCV)、艾滋病病毒抗体(抗-HIV)和梅毒螺旋体抗体(抗-TP)不合格情况,为制定安全血源招募策略和血液筛查反应性献血者归队提供依据。方法:采用酶联免疫吸附试验(ELISA)和核酸检测技术(NAT)对湖州地区2017年1月—2019年12月献血者进行HBsAg、抗-HCV、抗-HIV和抗-TP检测,如ELISA有反应性而NAT阴性,则进行相应的确证试验确认。对确认为真阳性的无偿献血者进行人口统计学分析,对HBsAg、抗-HCV、抗-HIV和抗-TP不合格情况分析。结果:HBsAg、抗-HCV、抗-HIV和抗-TP不合格率分别为0.33%、0.09%、0.06%和0.20%。HBsAg真阳性无偿献血者分别在献血次数、年龄和文化程度方面差异有统计学意义(P<0.01);抗-HCV真阳性无偿献血者分别在献血次数和性别方面差异有统计学意义(P<0.05);抗-HIV真阳性无偿献血者在献血次数方面差异有统计学意义(P<0.05);抗-TP真阳性无偿献血者分别在献血次数、年龄和文化程度方面差异有统计学意义(P<0.05)。HBsAg、抗-HCV、抗-HIV和抗-TP ELISA双试剂阳性与相对应单试剂阳性之间差异均有统计学意义(P<0.01)。HBsAg、抗-HCV、抗-HIV和抗-TP不合格标本ELISA结果在不同分组之间差异均有统计学意义(P<0.01)。结论:经确证试验可以有效降低献血者假阳性的产生。在献血招募工作中,重点招募年龄在18~30岁、学历专科及以上的重复献血者;主动召回单试剂阳性且S/CO值<3的献血者进入归队程序,减少血液资源浪费并为归队的献血者解除自身健康问题的困扰。Abstract: Objective: To analyze the unqualified condition of HBsAg, anti-HCV, anti-HIV and anti-TP of unpaid blood donors, and to provide a basis for formulating safe blood source recruitment strategies and blood screening of reactive blood donors.Methods: ELISA and NAT were used to detected HBsAg, anti-HCV, anti-HIV and anti-TP of unpaid blood donors in Huzhou area from January 2017 to December 2019. When the ELISA was positive but the NAT was negative, the corresponding confirmation experiment was performed. The disqualifications of HBsAg, anti-HCV, anti-HIV and anti-TP were analyzed, and the true positive blood donors were demographically analyzed.Results: The unqualified rates of HBsAg, anti-HCV, anti-HIV and anti-TP were 0.33%, 0.09%, 0.06% and 0.20%, respectively. There were statistically significant differences in HBsAg, anti-HCV, anti-HIV and anti-TP ELISA between the two positive agents and the corresponding single positive agents(P<0.01). There were statistically significant differences among HBsAg positive unpaid blood donors in the frequency of blood donations, age, and education level(P<0.01). There were statistically significant differences in the frequency of blood donation and gender among unpaid blood donors with true anti-HCV positive(P<0.05). The difference of anti-HIV positive unpaid blood donors in the frequency of blood donation was statistically significant(P<0.05). There were statistically significant differences among TP positive unpaid blood donors in the frequency of blood donation, age and educational level(P<0.05). ELISA results of HBsAg, anti-HCV, anti-HIV and anti-TP unqualified samples were statistically significant among different groups(P<0.01).Conclusion: The results of this study show that confirmation experiments can effectively reduce the false positive rate. In the blood donation recruitment work, the focus is on recruiting re-donors aged between 18 and 30 years old and college degree or above; actively recall blood donors with positive single reagents and S/CO values<3 into the reunification process to reduce waste of blood resources and relieve their health problems.
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Key words:
- HBsAg /
- anti-HCV /
- anti-HIV /
- anti-TP /
- unqualified rate
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