剂量效应在不规则抗体鉴定的应用分析——附1例报告

熊玉琪; 邹昕; 虞茜; 杨红梅

doi:10.13201/j.issn.1004-2806.2022.08.013

剂量效应在不规则抗体鉴定的应用分析——附1例报告

- 常州市中心血站(江苏常州，213000)
基金项目:
2021年常州市卫健委青年人才科技项目(No：QN202133)

详细信息

通讯作者: 杨红梅，E-mail：yhmei83@126.com

中图分类号: R457.1

收稿日期: 2022-02-10

修回日期: 2022-04-05

刊出日期: 2022-08-01

Application of dose effect in identification of irregular antibodies--a case report

- Changzhou Central Blood Station, Changzhou, 213000, China

More Information

Corresponding author: YANG Hongmei, E-mail: yhmei83@126.com

Received Date: 10 February 2022

Revised Date: 05 April 2022

Publish Date: 01 August 2022

摘要

摘要: 目的应用剂量效应分析抗-Ce联合抗-JK^a抗体引起的疑难配血。方法对患者进行ABO及Rh血型鉴定，Coombs试验进行抗体筛查，在盐水、抗人球蛋白和凝聚胺介质采用两套谱细胞进行不规则抗体鉴定及不规则抗体效价的测定。结果两套谱细胞反应格局结果相同，患者血清存在抗-Ce和抗-Jk^a抗体。结论剂量效应在Rh和Kidd血型系统不规则抗鉴定中有重要应用价值，抗-Ce和抗-Jk^a抗体引起患者配血困难。
- 剂量效应 /
- 抗-Ce /
- 抗-JK^a /
- 多谱细胞应用
Abstract: Objective To analyze the difficult blood matching caused by anti-Ce combined with anti-JK^aantibody by dose effect.Methods ABO and Rh blood typing, Coombs test for antibody screening, irregular antibodies were identified by two sets of panel cells in saline, anti human globulin and condensed amine medium, determination of irregular antibody titer.Results The results of the two sets of panel cell were the same, there were anti-Ce and anti-JK^aantibodies in the patient's serum.Conclusion Dose effect may have an important application value in irregular antibody identification of Rh and Kidd blood group system. Anti-Ce and anti-JK^aantibodies could cause difficulty in blood matching.
- dose effect /
- anti-Ce /
- anti-JK^a /
- muti panel cells application

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表 1 患者间接抗人球试验结果

序号	Rh-hr	D	C	E	c	e	C^w	K	k	M	N	S	s	P₁	Le^a	Le^b	Jk^a	Jk^b	Di^a	Kp^a	Kp^b	Js^a	Js^b	Lu^a	Lu^b	IS	poly	IAT
1	R₁R₁^W	+	+	0	0	+	+	0	+	0	+	+	+	+	+	0	+	+	0	0	+	n.t.	n.t.	0	+	0	2+^S	2+
2	R₂R₂	+	0	+	+	0	0	0	+	+	0	0	0	+	0	+	+	0	0	0	+	n.t.	n.t.	0	+	0	0	1+^W
3	rrK+^*	0	0	0	+	+	0	+	+	+	+	0	+	0	0	+	0	+	0	0	+	n.t.	n.t.	+	+	0	2+^S	1+
注：^*Co^b+。

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表 2 患者谱细胞1反应格局

序号	Rh-hr	D	C	E	c	e	C^w	K	k	M	N	S	s	P₁	Le^a	Le^b	Jk^a	Jk^b	Di^a	Kp^a	Kp^b	Js^a	Js^b	Lu^a	Lu^b	IS	poly	IAT
1	R₁R₁K+	+	+	0	0	+	0	+	0	+	+	+	0	0	0	+	+	+	0	0	+	n.t.	n.t.	0	+	0	2+^S	2+^S
2	R₂R₂	+	0	+	+	0	0	0	+	+	0	0	+	+	0	+	+	0	0	0	+	n.t.	n.t.	0	+	0	0	1+^W
3	r’r	0	+	0	+	+	0	0	+	+	+	0	+	0	0	0	+	+	0	0	+	n.t.	n.t.	0	+	0	2+^S	2+^S
4	r''r	0	0	+	+	+	0	0	+	+	0	+	+	0	0	+	+	+	0	0	+	n.t.	n.t.	0	+	0	2+	1+
5	rrK+	0	0	0	+	+	0	+	+	+	+	+	+	+	0	+	0	+	0	0	+	n.t.	n.t.	+	+	0	2+	2+^S
6	R₀r	+	0	0	+	+	0	0	+	0	+	0	+	+	0	+	+	0	0	0	+	n.t.	n.t.	0	+	0	2+	2+
7	R₁R₁^W	+	+	0	0	+	+	0	+	+	+	+	+	+	0	+	0	+	0	0	+	n.t.	n.t.	+	+	0	2+^S	2+^S
8	rr	0	0	0	+	+	0	0	+	+	+	0	+	+	+	0	0	+	0	0	+	n.t.	n.t.	0	+	0	1+^S	1+
9	R₁R₂	+	+	+	+	+	0	0	+	0	+	+	+	+	0	+	+	0	0	0	+	n.t.	n.t.	0	+	0	1+^S	2+^S
10	R₁R₁	+	+	0	0	+	0	0	+	+	0	0	+	+	+	0	0	+	0	+	+	n.t.	n.t.	0	+	0	2+^S	2+^S
11	rr	0	0	0	+	+	0	0	+	+	0	+	0	+	0	0	+	+	0	0	+	n.t.	n.t.	0	+	0	1+^S	2+^S
自c																										0	0	0

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表 3 患者谱细胞2反应格局

序号	Rh-Hr								Kell						Duffy		Kidd		Lewis		P	MNS				Luther		Xg	试管法
序号	C	D	E	c	e	C^W	f	V	K	k	Kp^a	Kp^b	Js^a	Js^b	Fy^a	Fy^b	Jk^a	Jk^b	Le^a	Le^b	P1	M	N	S	s	Lu^a	Lu^b	Xg^a	IS	IAT	poly
1	+	+	0	0	+	+	/	/	0	+	0	+	/	+	+	0	+	0	0	+	+	+	0	0	+	0	+	+	0	3+	3+^S
2	+	+	0	0	+	0	/	/	+	+	0	+	0	+	0	+	0	+	0	+	+	+	+	+	0	0	+	/	0	3+	3+^S
3	0	+	+	+	0	0	/	/	+	+	0	+	0	+	+	+	0	+	+	0	+	0	+	+	+	0	W	/	0	0	0
4	0	+	0	+	+	0	/	/	0	+	0	+	/	+	+	0	+	0	0	+	+	+	0	+	0	+	+	+	0	3+^W	3+^S
5	+	0	0	0	+	0	/	/	0	+	0	+	/	+	+	0	+	+	0	+	+	0	+	+	+	0	+	+	0	3+	3+^S
6	0	0	+	+	0	0	/	/	0	+	0	+	/	+	0	+	+	+	0	+	+	+	0	+	0	0	+	+	0	1+	0
7	0	0	0	+	+	0	/	/	0	+	0	+	0	+	+	0	+	0	0	0	0	+	+	0	+	0	+	/	0	3+	3+
8	0	0	0	+	+	0	/	/	0	+	+	+	0	+	+	0	0	+	+	+	+	+	+	0	+	0	+	/	0	3+^W	3+
9	0	0	0	+	+	0	/	/	+	0	0	+	/	+	0	+	0	+	+	0	+	+	+	0	+	0	+	+	0	2+	3+^S
10	0	0	0	+	+	0	/	/	0	+	0	+	0	+	+	+	+	0	0	+	+	0	+	+	0	0	+	/	0	3+	3+^S
11	+	+	+	0	+	+	/	/	0	+	0	+	0	+	0	+	+	+	+	0	+	+	+	0	+	0	+	/	0	2+	3+
12	W	+	+	+	0	0	/	/	+	+	0	+	/	+	+	0	+	+	+	0	+	0	+	0	+	0	+	/	0	1+	0
13	0	0	0	+	+	0	/	/	0	+	0	+	/	+	0	+	0	+	0	+	+	0	+	0	+	+	+	+	0	3+	3+
14	+	0	0	+	+	+	/	/	0	+	0	+	0	+	0	+	0	+	0	+	+	+	+	0	+	0	+	0	0	3+	3+
15	0	+	+	+	+	0	/	/	0	+	0	+	/	+	+	+	+	+	0	+	0	+	+	+	+	+	0	+	0	1+^S	3+
16	+	0	0	+	+	0	/	/	0	+	+	+	/	+	0	+	+	+	0	+	+	+	+	0	+	0	+	+	0	3+	3+
自c																													0	0	0

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