Study on distribution of HLA-A, B and HPA1-18, 21 gene polymorphisms in Han population in Jinan by melting curve analysis
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摘要: 目的探讨济南地区汉族人群血小板抗原(HPA)1-18，21和人类白细胞抗原(HLA-A、B)的基因多态性分布，为建立本地区机采血小板献血者基因分型资料库奠定基础。方法采用荧光PCR熔解曲线分析法对本站随机选取的347例无亲缘关系固定献血者进行HLA-A、B和HPA1-18，21基因分型。结果HLA-A、B位点分别检测出18个和34个等位基因，基因频率较高的分布为A*02(0.278 1)，A*11(0.168 6)，A*24(0.147 0)与B*13(0.142 7)、B*60(0.069 2)、B*61(0.064 8)、B*62(0.063 4)。最常见的HPA基因组合型为：HPA-1a/1a-2a/2a-3a/3b-4a/4a-5a/5a-6a/6a-(7-14)a/a-15a/15b-(16-18)a/a-21a/21a。结论济南汉族人群HLA和HPA分布具有多态性，与其他地区和种族比较有差异性，应建立本地区血小板捐献者的HLA与HPA基因分型资料库。Abstract: ObjectiveTo investigate the gene polymorphism distribution of platelet antigen(HPA) 1-18, 21 and human leukocyte antigen(HLA-A, B) in Han population in Jinan, so as to lay a foundation for establishing the genotyping database of mechanically collected platelet donors in this area.MethodsThe HLA-A, B and HPA1-18, 21 genotypes of 347 unrelated fixed blood donors were analyzed by fluorescence PCR fusion curve analysis.Results18 alleles and 34 alleles were detected at HLA-A and B loci respectively. The higher gene frequencies were A*02(0.278 1), A*11(0.168 6), A*24(0.147 0) and B*13(0.142 7), B*60(0.069 2), B*61(0.064 8) and B*62(0.063 4). The most common HPA genotypes were: HPA-1a / 1a-2a / 2a-3a / 3b-4a / 4a-5a / 5a-6a / 6A-(7-14) a / a-15a / 15b-(16-18) a / a-21a / 21a.ConclusionThe distribution of HLA and HPA in Jinan Han population was polymorphic, which was different from other regions and races. The HLA and HPA genotyping database of platelet donors in this region should be established.
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表 1 济南地区347例血小板捐献者HLA-A、B位点等位基因频率
等位基因 观察值 基因频率 等位基因 观察值 基因频率 等位基因 观察值 基因频率 A*01 29 0.041 8 B*07 23 0.033 1 B*52 35 0.050 4 A*02 193 0.278 1 B*08 3 0.004 3 B*54 18 0.025 9 A*03 29 0.041 8 B*13 99 0.142 7 B*55 14 0.020 2 A*11 117 0.168 6 B*18 2 0.002 9 B*56 1 0.001 4 A*23 2 0.002 9 B*27 21 0.030 3 B*57 10 0.014 4 A*24 102 0.147 0 B*35 30 0.043 2 B*58 26 0.037 5 A*26 17 0.024 5 B*37 8 0.011 5 B*60 48 0.069 2 A*29 10 0.014 4 B*38 12 0.017 3 B*61 45 0.064 8 A*30 73 0.105 2 B*39 14 0.020 2 B*62 44 0.063 4 A*31 31 0.044 7 B*3901 8 0.011 5 B*63 1 0.001 4 A*32 14 0.020 2 B*41 1 0.001 4 B*65 2 0.002 9 A*33 49 0.070 6 B*44 33 0.047 6 B*67 13 0.018 7 A*34 2 0.002 9 B*46 41 0.059 1 B*71 13 0.018 7 A*66 1 0.001 4 B*48 39 0.056 2 B*72 1 0.001 4 A*68 8 0.011 5 B*49 2 0.002 9 B*75 38 0.054 8 A*203 8 0.011 5 B*50 7 0.010 1 B*81 1 0.001 4 A*210 6 0.008 6 B*51 37 0.053 3 A*2403 3 0.004 3 B*5102 4 0.005 8 
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表 2 济南地区347例血小板捐献者HPA基因型和基因频率分布
基因型频率 基因频率 P(H-W平衡) MMP aa ab bb a b HPA-1 0.988 5 0.0115 0 0.994 2 0.005 8 0.137 0.011 4 HPA-2 0.887 6 0.109 5 0.002 9 0.942 4 0.057 6 1 0.102 7 HPA-3 0.314 1 0.510 1 0.175 8 0.569 2 0.430 8 0.881 0.370 2 HPA-4 0.994 2 0.005 8 0 0.997 1 0.002 9 1.000 0 0.005 7 HPA-5 0.968 3 0.031 7 0 0.984 1 0.015 9 1.000 0 0.030 7 HPA-6 0.979 8 0.020 2 0 0.989 9 0.010 1 1.000 0 0.019 8 HPA-7 1.000 0 0 0 1.000 0 0 NA 0 HPA-8 1.000 0 0 0 1.000 0 0 NA 0 HPA-9 1.000 0 0 0 1.000 0 0 NA 0 HPA-10 1.000 0 0 0 1.000 0 0 NA 0 HPA-11 1.000 0 0 0 1.000 0 0 NA 0 HPA-12 1.000 0 0 0 1.000 0 0 NA 0 HPA-13 1.000 0 0 0 1.000 0 0 NA 0 HPA-14 1.000 0 0 0 1.000 0 0 NA 0 HPA-15 0.322 8 0.440 9 0.236 3 0.543 2 0.456 8 0.329 0.373 1 HPA-16 1.000 0 0 0 1.000 0 0 NA 0 HPA-17 1.000 0 0 0 1.000 0 0 NA 0 HPA-18 1.000 0 0 0 1.000 0 0 NA 0 HPA-21 0.979 8 0.020 2 0 0.989 9 0.010 1 1.000 0 0.019 8
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[1] 贺锋, 李彦勋, 程金凤. 免疫性血小板减少性紫癜患者血小板输注疗效影响因素分析[J]. 临床血液学杂志, 2020, 33(12): 825-828. http://lcxz.cbpt.cnki.net/WKC/WebPublication/paperDigest.aspx?paperID=a9c0c0e9-51cd-40cd-8a83-feb753452438
[2] 张忠印, 吉飞跃, 周小玉. HLA-Ⅰ抗体及血小板膜糖蛋白CD41a的表达与血小板输注无效的相关性及影响因素分析[J]. 临床血液学杂志, 2021, 34(8): 545-547, 551. http://lcxz.cbpt.cnki.net/WKC/WebPublication/paperDigest.aspx?paperID=b8a9c670-164f-4725-88cd-51997a666e61
[3] 杜飞嫦, 洪文旭, 吴凡, 等. 稀有血型库和血小板供者资料库的研究进展[J]. 检验医学与临床, 2021, 18(2): 276-278. doi: 10.3969/j.issn.1672-9455.2021.02.041
[4] 王超, 胡晓玉, 赵阳, 等. 安徽省汉族人群HLA-A、-B、-C、-DRB1、-DQB1等位基因分布及单体型多态性研究[J]. 中国输血杂志, 2021, 34(3): 240-244. https://www.cnki.com.cn/Article/CJFDTOTAL-BLOO202103013.htm
[5] 苏湘晖, 孙昂, 粟玉萍, 等. 岳阳地区无偿单采血小板捐献现状调查和血小板供者库扩容[J]. 中国误诊学杂志, 2019, 14(4): 148-150. https://www.cnki.com.cn/Article/CJFDTOTAL-ZWZX201904002.htm
[6] 许飞, 贺晨峰, 曹锁春, 等. 常州地区单采血小板捐献者HLA抗原分布情况[J]. 临床血液学杂志, 2018, 31(4): 294-296. https://www.cnki.com.cn/Article/CJFDTOTAL-LCXZ201804014.htm
[7] 吕启露, 廖秀云, 纪欣, 等. 四川德阳地区血小板捐献者HPA-1~6w、HPA-15及HPA-32bw~35bw多态性研究[J]. 中国输血杂志, 2022, 35(2): 149-153. https://www.cnki.com.cn/Article/CJFDTOTAL-BLOO202202005.htm
[8] 刘孟黎, 齐珺, 沈春梅, 等. 中国西藏藏族人群HLA-A/B/DRB1座位基因遗传多态性和单体型分析[J]. 中国输血杂志, 2011, 24(2): 96-100. https://www.cnki.com.cn/Article/CJFDTOTAL-BLOO201102007.htm
[9] 苏蔓, 李茵, 赵倩, 等. 石家庄地区已知HLA型别单采血小板捐献者基因资料库库容评价[J]. 临床输血与检验, 2020, 22(3): 290-294. https://www.cnki.com.cn/Article/CJFDTOTAL-LSXY202003016.htm
[10] Shen CM, Zhu BF, Li SB. [HLA-A, B, and DRB1 gene polymorphisms in Mongol ethnic group of Inner Mongolia, China][J]. Yi Chuan, 2008, 30(2): 164-168.
[11] Foxcroft ZK, Schlebusch CM. Human Platelet Antigen(Hpa)1-5 And-15 Frequencies In South African Blood Donors[J]. Africa Sanguine, 2008, 11(1): 15-18.
[12] 薛敏, 潘芹芹, 樊甦, 等. 已知HLA单采血小板供者资料库库容估计[J]. 中国实验血液学杂志, 2019, 27(1): 266-271. https://www.cnki.com.cn/Article/CJFDTOTAL-XYSY201901046.htm
[13] Bennett JA, Palmer LJ, Musk AW, et al. Gene frequencies of human platelet antigens 1-5 in indigenous Australians in Western Australia[J]. Transfus Med, 2002, 12(3): 199-203.
[14] 刘瑛, 许先国, 马开荣, 等. HLA Ⅰ基因型配合输注策略用于免疫性血小板输注无效的实验研究[J]. 中国输血杂志, 2021, 34(8): 832-835. https://www.cnki.com.cn/Article/CJFDTOTAL-BLOO202108007.htm
[15] 王晓伟, 邵春燕, 张菁, 等. 北京地区HPA1-6,15基因库的建立与应用[J]. 中国实验血液学杂志, 2020, 28(1): 296-299. https://www.cnki.com.cn/Article/CJFDTOTAL-XYSY202001055.htm
[16] Seike K, Fujii N, Asano N, et al. Efficacy of HLA virtual cross-matched platelet transfusions for platelet transfusion refractoriness in hematopoietic stem cell transplantation[J]. Transfusion, 2020, 60(3): 473-478.
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