Study on the expression and clinical characteristics of micro ribonucleic acid in neonates with congenital heart disease
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摘要: 目的 研究新生儿先天性心脏病微小RNA-222-3p(miR-222-3p)、微小RNA-1(miR-1)、微小RNA-324-5p(miR-324-5p)表达及与临床特征的相关性。方法 选取2020年3月至2021年3月收治的88例先天性心脏病新生患儿作为疾病组，另选取同期健康体检新生儿50例作为对照组，采用荧光定量PCR法检测所有研究对象miR-222-3p、miR-324-5p、miR-1表达量。结果 与对照组比较，疾病组miR-222-3p、miR-1、miR-324-5p表达均显著升高，有肺动脉狭窄、主动脉狭窄、房间隔缺损患儿miR-222-3p、miR-1、miR-324-5p表达量升高，预后不良组miR-222-3p、miR-1、miR-324-5p表达均显著上升，与miR-1、miR-222-3p、miR-324-5p比较，3项联合对疾病组患儿预测及诊断价值较高，差异有统计学意义(P < 0.05)。miR-222-3p、miR-1、miR-324-5P的相关性分析显示，miR-222-3p与miR-1呈正相关(r=0.269，P=0.018)；miR-1与miR-324-5p呈正相关(r=0.245，P=0.034)；miR-222-3p与miR-324-5P呈正相关(r=0.329，P=0.002)。结论 miR-222-3p、miR-1、miR-324-5p在新生儿先天性心脏病中异常高表达，可用于临床上新生儿先天性心脏病的检测。
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关键词:
- 新生儿先天性心脏病 /
- 微小RNA-222-3p /
- 微小RNA-1 /
- 微小RNA-324-5p /
- 临床特征
Abstract: Objective To study the expression of microRNA-222-3p(miR-222-3p), microRNA-1(miR-1) and microRNA-324-5p(miR-324-5p) and their correlation with clinical characteristics in neonatal congenital heart disease.Methods A total of 88 neonatal children with congenital heart disease admitted to our hospital from March 2020 to March 2021 were selected as the disease group, and 50 healthy volunteers were selected for health examination in our hospital in the same period. All the expression levels of miR-222-3p, miR-1 and miR-324-5p were measured by quantitative PCR method.Results Compared with the healthy group, the expression levels of miR-222-3p, miR-1 and miR-324-5p were all significantly increased in the disease group, the expressions of miR-222-3p, miR-324-5p and miR-1 in the children with pulmonary stenosis, aortic stenosis and atrial septal defects were all elevated, the expressions of miR-222-3p, miR-1 and miR-324-5p were all significantly increased in the group with poor prognosis. In contrast to the miR-222-3p, miR-1 and miR-324-5p, the three combinations had a high predictive value of poor prognosis for children in the disease group, The difference was statistically significant(P < 0.05). Correlations between miR-222-3p, miR-1 and miR-324-5p showed a positive correlation between miR-222-3p and miR-1(r=0.269, P=0.018), a positive correlation between miR-222-3p and miR-324-5p(r=0.329, P=0.002), and a positive correlation between miR-1 and miR-324-5p(r=0.245, P=0.034).Conclusion The miR-222-3p, miR-1, and miR-324-5p were abnormally highly expressed in neonatal congenital heart disease, and could be used clinically in the detection of neonatal congenital heart disease.-
Key words:
- neonates with congenital heart disease /
- miR-222-3p /
- miR-1 /
- miR-324-5p /
- clinical features
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表 1 miR-222-3p、miR-1、miR-324-5p在疾病组和对照组中表达比较
X±S 组别 例数 miR-222-3p miR-1 miR-324-5p 疾病组 88 1.68±0.15 0.59±0.05 1.87±0.18 对照组 50 0.21±0.02 0.10±0.01 0.11±0.01 t 68.840 68.420 68.870 P 0.001 0.001 0.001 
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表 2 miR-222-3p、miR-1、miR-324-5p表达与疾病组患儿临床特征的相关性分析
X±S 项目 例数 miR-222-3 t P miR-1 t P miR-324-5p t P 性别 0.567 0.001 0.850 0.001 0.253 0.001 男 43 1.69±0.16 0.60±0.06 1.88±0.19 女 45 1.71±0.17 0.59±0.05 1.87±0.18 肺动脉狭窄 40.000 0.001 40.420 0.001 44.490 0.001 有 65 1.73±0.18 0.63±0.06 1.91±0.19 无 23 0.22±0.02 0.12±0.01 0.14±0.01 主动脉狭窄 33.010 0.001 23.070 0.001 36.510 0.001 有 73 1.76±0.17 0.65±0.06 1.93±0.19 无 15 0.30±0.03 0.14±0.01 0.13±0.01 房间隔缺损 436.700 0.001 42.950 0.001 45.710 0.001 有 62 1.78±0.17 0.67±0.06 1.95±0.20 无 26 0.31±0.03 0.16±0.01 0.15±0.01
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表 3 miR-222-3p、miR-1、miR-324-5p与疾病组患儿发生不良预后的关系
X±S 组别 例数 miR-222-3p miR-1 miR-324-5p 预后良好组 76 1.34±0.13 0.18±0.01 0.26±0.02 预后不良组 12 2.91±0.29 1.96±0.19 2.57±0.25 t 31.650 83.540 81.420 P 0.001 0.001 0.001
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表 4 miR-222-3p、miR-1、miR-324-5p对疾病组患儿不良预后的预测价值分析
项目 AUC 95%CI 灵敏度/% 特异度/% 准确度/% P miR-222-3p 0.719 0.701~0.732 85.73 85.45 81.93 0.007 miR-1 0.740 0.731~0.847 89.31 73.21 86.73 0.006 miR-324-5p 0.735 0.721~0.741 90.08 70.19 84.32 0.008 三项联合 0.941 0.932~0.959 99.46 68.01 89.78 0.005
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