Effects of different storage conditions and different sample types on concentration analysis of sCD40L in blood samples
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摘要: 目的 探究不同储存条件及不同样本类型对血样中可溶性白细胞分化抗原CD40配体(sCD40L)浓度的影响，为临床更精确检测sCD40L提供保障。方法 随机选取正常人的血浆和血清标本，分别在4℃和室温下储存0、2、4、8、16、24、36、48 h，利用ELISA方法检测其中sCD40L的表达水平。结果 室温及4℃条件下，血清中sCD40L浓度显著高于其血浆中sCD40L浓度(P＜0.05)；4℃条件下，同一检测者血浆和血清中sCD40L浓度随放置时间变化差异无统计学意义(P>0.05)；室温条件下，血浆内sCD40L浓度随放置时间延长而升高，血清中sCD40L浓度随时间变化先升高再降低。结论 sCD40L作为人体一种重要的炎性因子，在其精确检测的过程中，要确定待检标本是血清或血浆及不同的储存时间。Abstract: Objective To explore the effects of different storage conditions and different sample types on the concentration of soluble leukocyte differentiation antigen CD40Ligand(sCD40L) in blood samples, so as to provide guarantee for more accurate clinical detection of sCD40L.Methods Normal plasma and serum samples were randomly selected and stored at 4℃ and room temperature for 0, 2, 4, 8, 16, 24, 36, 48 h, respectively. The expression level of sCD40L was detected by ELISA.Results At room temperature and 4℃, serum sCD40L concentration was significantly higher than plasma sCD40L concentration(P < 0.05). At 4℃, sCD40L concentration in plasma and serum of the same test subjects had no significant difference with the time of placement(P>0.05). At room temperature, plasma sCD40L concentration increased with time, while serum sCD40L concentration first increased and then decreased with time.Conclusion sCD40L is an important inflammatory factor in human body. In the process of its accurate detection, it is necessary to determine whether the specimen to be tested is serum or plasma and the different storage time.
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Key words:
- sCD40L /
- platelets /
- ELISA /
- concentration analysis
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表 1 不同温度储存条件下，血浆及血清中sCD40L(测量A值)随时间变化差异
X±S 不同时间 室温保存血浆标本 室温保存血清标本 4℃保存血浆标本 4℃保存血清标本 对照组(0 h) 0.015±0.003 0.448±0.090 0.015±0.003 0.448±0.090 2 h 0.022±0.008 0.574±0.076 0.017±0.004 0.475±0.144 4 h 0.047±0.0131) 0.625±0.107 0.017±0.002 0.484±0.101 6 h 0.097±0.0151) 0.652±0.102 0.017±0.003 0.461±0.098 8 h 0.133±0.0131) 0.603±0.111 0.016±0.001 0.489±0.109 12 h 0.148±0.0061) 0.512±0.062 0.018±0.004 0.454±0.095 24 h 0.166±0.0101) 0.486±0.079 0.018±0.003 0.457±0.057 36 h 0.189±0.0031) 0.362±0.101 0.018±0.001 0.473±0.050 48 h 0.202±0.0121) 0.292±0.041 0.018±0.003 0.472±0.067 与对照组比较，1)P＜0.05。 
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