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摘要: 此文患者因进行输血前检测时出现正反定型不符，遂通过血清学方法对患者血样进行血型鉴定、抗体筛选鉴定、交叉配血等实验。观察患者血清在不同温度下与红细胞的反应，发现血清在常温下与A、O型红细胞凝集强度分别为w+、4+，在37℃时与A、O型红细胞无凝集；抗体筛查实验，在22℃盐水介质下，与Ⅰ、Ⅱ、Ⅲ号细胞均有凝集，37℃时均无凝集；在室温时与谱细胞凝集阳性，37℃下与谱细胞凝集阴性；在室温下与成人O型红细胞反应4+，与成人A型红细胞反应1+，与脐带血红细胞无凝集反应。确定患者血浆中含有抗-HI抗体。Abstract: In this study, a patient presented with ABO blood group discrepancy during pre-transfusion testing. Serological methods were employed to identify the patient's blood type, antibody screening and crossmatching experiments were done. The patient's serum reactivity with red blood cells at different temperatures was observed. It was found that the serum agglutinated strongly with A and O type red blood cells at room temperature(w+ and 4+ reactions, respectively) but did not agglutinate at 37℃. Antibody screening experiments in a saline medium at 22℃ resulted in agglutination with cells Ⅰ, Ⅱ and Ⅲ, while no agglutination occurred at 37℃. Furthermore, agglutination was positive with panel cells at room temperature but negative at 37℃. The patient's serum reacted with adult O type red blood cells at a 4+ level, with adult A type red blood cells at a 1+ level, and showed no reactivity with cord blood red cells. These confirmed the presence of anti-HI antibodies in the patient's plasma.
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Key words:
- anti-HI /
- antibody /
- blood typing
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表 2 患者血浆与抗体筛选细胞反应格局
序号 Rh-hr Kidd MNS Duffy Lewis P 患者 D C E c e Jka Jkb M N S s Fya Fyb Lea Leb P1 NS 22℃ AHG 37℃ 1 + 0 + + 0 + 0 + 0 0 + + + 0 + 0 4+ 0 2 + + 0 0 + + + + + 0 + + 0 + + 0 4+ 0 3 + + + + + 0 + + 0 + + + 0 + + + 4+ 0 
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表 3 患者血浆与谱细胞反应格局
谱细胞 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 22℃ 4+ 3+ 4+ 4+ 3+ 4+ 3+ 4+ 4+ 3+ 4+ 4+ 4+ 4+ 3+ 4+ 37℃ 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
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| 引用本文: |
赵薇, 杨宜承, 霍林红, 等. 抗-HI抗体导致正反定型不符1例[J]. 临床血液学杂志, 2023, 36(12): 906-908. doi: 10.13201/j.issn.1004-2806.2023.12.014
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| Citation: |
ZHAO Wei, YANG Yicheng, HUO Linhong, et al. One case of positive and negative blood typing discrepancy caused by anti-HI[J]. J Clin Hematol, 2023, 36(12): 906-908. doi: 10.13201/j.issn.1004-2806.2023.12.014
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