Rh系统简单特异性自身抗体血清学鉴定与分析

张刘仟; 马玲; 邵雷; 臧炫月; 尤建国

doi:10.13201/j.issn.1004-2806.2024.08.013

Rh系统简单特异性自身抗体血清学鉴定与分析

- 1.
  淮安市第一人民医院输血科(江苏淮安，223300)
- 2.
  江苏省血液中心输血研究室

详细信息

通讯作者: 尤建国，E-mail：youjg1968@163.com

中图分类号: R457.1

收稿日期: 2023-10-13

修回日期: 2024-06-26

刊出日期: 2024-08-01

Serological identification and analysis of simple Rh-specific autoantibodies

- 1.
  Department of Blood Transfusion, Huai'an First People's Hospital, Huai'an, 223300, China
- 2.
  Research Laboratory for Transfusion, Jiangsu Province Blood Center

More Information

Corresponding author: YOU Jianguo, E-mail: youjg1968@163.com

Received Date: 13 October 2023

Revised Date: 26 June 2024

Publish Date: 01 August 2024

摘要

摘要: 目的通过2例Rh系统简单特异性自身抗-e抗体案例的鉴定和分析，了解简单特异性自身抗体的血清学特征。方法采用常规血清学方法对患者进行ABO、Rh表型分型，直接抗人球蛋白试验对患者血清及放散液中抗体进行检测；进一步采用不同表型细胞对患者血清吸收后进行抗体鉴定，并对吸收后细胞再次放散检测。结果患者均为e抗原阳性，血清和放散液中表现出抗-e抗体格局，该抗体只能被e抗原阳性细胞吸收，而不能被e抗原阴性细胞吸收。结论该2例抗体为简单特异性自身抗e抗体，不能被e抗原阴性细胞吸收，与类抗体特征存在区别。
- 简单特异性自身抗体 /
- 抗体鉴定 /
- 抗-e
Abstract: Objective To understand the serological characteristic of simple specific autoantibodies by studies of 2 patients who produced simple specific auto anti-e antibodies.Methods ABO, Rh phenotyping, DAT and antibody identification were conducted by routine serological methods. Antibody identification was done using different phenotype RBCs with the eluting solution and absorbed serum.Results Both 2 patients were e positive. The antibodies in serum and eluting solution were identified as anti-e which could not be absorbed by e negative RBCs.Conclusion These two antibodies were simple specific auto anti-e. It cannot be absorbed by e negative RBCs which distinguish with mimicking antibody.
- simple specific antibody /
- antibody identification /
- anti-e antibody

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表 1 患者血型及DAT结果

患者	正定型		反定型			Rh血型					DAT
患者	抗-A	抗-B	A1c	Bc	Oc	抗-D	抗-C	抗-c	抗-E	抗-e	抗-IgG	抗-C3d
患者1	4+	4+	0	0	0	3+	3+	0	0	4+	3+	0
患者2	4+	4+	0	0	0	4+	3+	0	0	3+	3+	0

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表 2 抗体鉴定结果

序号	Rh-hr								Kell								Duffy		Kidd
序号	C	D	E	c	e	C^w			K		k	Kp^a	Kp^b	Js^a	Js^b		Fy^a	Fy^b	Jk^a	Jk^b
1	+	+	0	0	+	+			0		+	0	+	/	+		+	0	+	0
2	+	+	0	0	+	0			+		+	0	+	/	+		0	+	+	+
3	0	+	+	+	0	0			0		+	0	+	0	+		+	0	+	0
4	0	+	0	+	+	0			0		+	0	+	0	+		0	0	+	0
5	+	0	0	0	+	0			0		+	0	+	/	+		+	+	+	+
6	0	0	+	+	0	0			0	+		0	+	/	+		+	0	+	+
7	0	0	0	+	+	0			+		0	0	+	/	+		0	+	+	+
8	0	0	0	+	+	0			0		+	+	+	0	+		0	+	0	+
9	0	0	0	+	+	0			+		+	0	+	0	+		+	+	+	0
10	0	0	0	+	+	0			0		+	0	+	/	+		+	0	w	0
11	+	+	+	0	+	0			+		+	0	+	/	+		+	+	0	+
自c

序号	Lewis			P		MNS					Luther			Xg		患者1			患者2
序号	Le^a	Le^b		P1		M	N	S	s		Lu^a	Lu^b		Xg^a		IS	IAT	放散液	IS	IAT	放散液
1	0	+		+		+	+	+	0		0	+		+		0	1+	3+^s	0	1+^w	2+
2	0	+		+		0	+	0	+		0	+		+		0	1+	3+^s	0	1+^w	2+
3	+	0		+		0	+	0	+		0	+		+		0	0	0	0	0	0
4	0	0		+		0	+	0	+		0	+		+		0	±	3+^s	0	±	2+^s
5	0	+		+		+	0	+	+		0	+		+		0	1+	3+^s	0	1+	2+
6	0	+		+		0	+	+	+		0	+		+		0	0	0	0	0	0
7	0	+		0		+	0	+	0		0	+		0		0	1+	3+^s	0	±	1+
8	+	0		+		+	+	0	+		0	+		+		0	1+	3+^s	0	±	2+^w
9	0	+		+		+	0	+	0		+	+		+		0	1+	3+^s	0	±	1+
10	+	0		+		+	0	+	+		0	+		+		0	1+^w	3+^s	0	±	2+^w
11	0	+		0		+	+	+	+		0	+		+		0	1+^w	3+^s	0	±	1+^s
自c																0	4+^w	/	0	2+^s	/

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表 3 吸收放散结果

序号	Rh-hr						ccDEE细胞吸收		酶处理ccDEE细胞吸收	CCDee细胞吸收
序号	C	D	E	c	e	C^w	吸收后血清	放散液	吸收后血清	吸收后血清	放散液
1	+	+	0	0	+	+	+	0	+	0	+
2	+	+	0	0	+	0	+	0	+	0	+
3	0	+	+	+	0	0	0	0	0	0	0
6	0	0	+	+	0	0	0	0	0	0	0
注：挑选谱细胞中e抗原阳性(1号、2号)及阴性(3号、6号)的细胞各2例进行抗体鉴定。

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