The correlations of pipette order and the sensitivity of antibodies testing with micro-column card and attach a case report
-
摘要: 目的:比较和分析在使用微柱凝胶卡检测的方法中,检测抗体或交叉配血的灵敏度、试剂与血浆或红细胞移液次序的相关性。方法:采用戴安娜微柱凝胶卡与达亚美微柱凝胶卡,测定两类不规则抗体效价,用评分方式对凝集结果进行判断比较其灵敏度,并对交叉配血实例进行评价。结果:两类微柱凝胶配血卡检测抗体效价时,先加血浆或试剂的检测结果均比先加红细胞的好。而在个例报道中确实存在低频抗原由于移液顺序而未被检出。结论:规范微柱凝胶配血卡移液先后顺序,能更有效地解决输血反应所产生的问题。Abstract: Objective:To realize the correlations of the sensitivity of antibody testing,cross-match and different pipette order of reagents,plasma and red blood cell in the micro-column gel card.Method:Assay the titers of the two types of irregular antibody with Diana micro-column gel card and Dimed micro-column gel card,with the way to score for judging the sensitivity and attach a case report.Result:With the two types of micro-column gel card test serum antibody titer,the results of plasma or reagent first are better than the results of red blood cells first.Conclusion:Regulate the pipette order with micro-column gel card,it can do more effective to solve the problems that clinicians are paying more and more attention to as transfusion reactions.
-
Key words:
- micro-column gel card /
- pipette order /
- anti-D /
- anti-E /
- antibody titers
-
-
[1] 刘达庄.免疫血液学[M].上海.上海科学技术出版社,2002:217-217.
[2] 于华,张春秀.抗原抗体之间的相互反应及其研究方法[J].化工时刊,2004,18(4):17-19.
[3] 沈关心,周汝麟.现代免疫学试验技术[M].2版.武汉:湖北科学技术出版社,2002:95-98.
[4] 丁琪,兰炯采.红细胞输注所致免疫反应的研究进展[J].临床血液学杂志(输血与检验版),2007,4(6):283-283.
[5] URBANIA K S J.Alloimmunity to RhD in humans[J].Transfus Clin Biol,2006,13:19-22.
[6] 陈秉宇,李育,沈健,等.献血员不完全抗体和直接抗人球蛋白阳性率的研究[J].检验医学,2007,22(4):502-504.
-
计量
- 文章访问数: 106
- PDF下载数: 158
- 施引文献: 0
下载: