海藻糖联合葡萄糖负载红细胞不同时间下溶血情况

姚根宏; 汪海蓉; 陈虎诚; 吴永政; 栾建凤; 朱培元; 叶东; 严京梅

海藻糖联合葡萄糖负载红细胞不同时间下溶血情况

- 1 南京军区南京总医院输血科(南京, 210002);
- 2南京大学金陵学院化学与环境生物科学系
基金项目:
南京军区医学科技创新资助项目(No:09MA087)

南京军区南京总医院科研基金(No:2011048)

详细信息

通讯作者: 姚根宏,E-mail:yaogenhong@sohu.com

中图分类号: R318.52

收稿日期: 2011-08-15

Hemolytic effect of trehalose and glucose loading red blood cells at different time

- 1. 1Department of Transfusion,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing,210002,China;
- 2. 2School of Life Science,Jinling Nanjing College of Jinling University

More Information

Corresponding author: YAO Genhong, E-mail: yaogenhong@sohu.com

Received Date: 15 August 2011

摘要

摘要: 目的:探索海藻糖和葡萄糖联合负载红细胞的最适时间,为冷冻干燥红细胞提供基础。方法:分别采用0、0.125、0.250、0.500和1.000 mol/L的海藻糖联合葡萄糖在37℃负载红细胞4、6和8 h。然后检测上清液中游离血红蛋白和乳酸脱氢酶含量。结果:负载液浓度为1 mol/L时,3种负载时间下的细胞溶血程度较重,即上清中游离血红蛋白和乳酸脱氢酶浓度较高。在浓度低于1 mol/L,负载时间为4、6 h时,上清中游离血红蛋白和乳酸脱氢酶浓度较低。而负载时间延长至8 h时,红细胞溶血程度较高。结论:在浓度小于1 mol/L时,海藻糖联合葡萄糖在37℃情况下负载红细胞6 h时对细胞的损伤较小,能够满足冻存要求。
- 红细胞保存 /
- 冷冻干燥 /
- 最适时间 /
- 海藻糖 /
- 葡萄糖
Abstract: Objective:To explore the optimum time of trehalose and glucose loading red blood cells for lyophilization. Method:The red blood cells were loaded with 0,0.125,0.250,0.500 and 1.000 mol/L trehalose and glucose respectively in 37℃ for 4,6,and 8 hours. Then,the levels of free hemoglobin and lactate dehydrogenase in the supernatants were determined. Result:The results showed that free hemoglobin and lactate dehydrogenase significantly increased in three groups of 1 mol/Ltrehalose and glucose(4,6,and 8 h). When red blood cells were loading with 0.125,0.250,and 0.500 mol/L trehalose and glucose for 4 and 6 h,the free hemoglobin and lactate dehydrogenase were low. However,when the incubation time extend to 8h(0.125,0.250,and 0.500 mol/L),the red blood cells showed significant hemolysis. Conclusion:These results suggested that loading with trehalose and glucose(0.125,0.250,and 0.500 mol/L)at 37℃ for 6h had little hemolytic effect on red blood cells and were qualified for loading red blood cells for purpose of lyphilization.
- human red blood cell preservation /
- Lyophilization /
- optimum time /
- Trehalose /
- Glucose

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