Comparison of automated capillary zone electrophoresis and high-performance liquid chromatography on quantitative study of hemoglobin
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摘要: 目的：探讨用法国Sebia公司Capillarys2全自动毛细管电泳（CZE）对血红蛋白定性与定量分析的准确性。方法：采集205例广东地区成人EDTA-K2抗凝血2 ml，分别采用高效液相色谱法（HPLC）和capillarys方法各自测定血红蛋白含量。结果：两种方法测定HbA2的准确度，在205例样本中，CZE测定有93%的测定结果与HPLC测定值的百分差异在±20%范围内，与95%相比较，P值为0.1944，差异无统计学意义。其中CZE测得的HbA2异常21例，HbF增高5例，Hb A2+HbF同时增高12例；用HPLC对这205例样本进行复检对照，结果基本一致。检测出HbH 7例，HbE 7例，Hb NewYork 5例，Hb G-thailand 3例，Hb Bart's 3例，HbS 2例。但是，对于HbA2的定量，用CZE测得的水平高于HPLC。同时，我们观察到CZE较HPLC对HbH和Hb Bart's的检测更为敏感。结论：HPLC和CZE都能能够分离和量化出HbA2和HbF异常增高，是一种快速、准确、可靠的筛查地中海海贫血实验方法，但是两者在鉴定异常血红蛋白时存在区别。Abstract: Objective: To compare automated capillary zone electrophoresis (CZE),the French Sebia company production,with high performance liquid chromatography (HPLC)in the accuracy of qualitative and quantitative analysis of hemoglobin.Method: 2 ml EDTA-K2 anti-coagulated blood samples were collected from 205 Guangdong adults.The hemoglobin content was detected by HPLC and CZE respectively.Result: Among 205 samples,93% of the results detected by CZE,was in ±20% difference with the results detected by HPLC,compared with 95%,p-value was 0.1944,there was no significant difference of statistical significance.Among the results,21 cases were detected by CZE with abnormal HbA2,15 cases with high HbF,12 cases with high HbA2 and HbF.The results were almost the same detected by HPLC.Two systems correctly identified a number of hemoglobin variants,7 cases with HbH,7 cases with HbE,5 cases with Hb NewYork,3 cases with Hb G-Thailand,3 cases with Hb Bart's and 2 cases with HbS.However,CZE detected result was higher than that of HPLC for HbA2 quantitative analysis.At the same time,CZE was more sensitive for HbH and Hb Bart's detection compared to HPLC.Conclusion: Both CZE and HPLC could separate and identify the value of HbA2 and HbF.The two experiment methods were quick and accurate on the screening of thalassemia and some hemoglobin diseases.But there were some differences on the detection of hemoglobin between them.
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Key words:
- automated capillary zone electrophoresis /
- HPLC /
- hemoglobin /
- thalassemia
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[1] OLIVIERI N F.The β-thalassemias[J].N Engl J Med, 1999, 341:99-109.
[2] JOINER C H.Universal newborn screening for hemoglobinopathies[J].J Pediatr, 2000, 136:145-146.
[3] WAJCMAN H, PREHU C, BARDAKDJIAN-MICHAU J, et al.Abnormal hemoglobins:laboratory methods[J].Hemoglobin, 2001, 25:169-181.
[4] LOUAHABIA, PHILIPPEM, LAUS, et al.Evaluation of a new sebia kit for analysis of hemoglobin fractions and variants on the capillarys system[J].Clin Chem Lab Med, 2006, 44:340-345.
[5] WEATHERALL D J.The thalassemias[M]//BEUTLER E, LICHTMAN M A, COLLER B S, et al.Williams hematology.New York:McGrawHill, 1995:581-615.
[6] BEUTLER E.The sickle cell disease and related disorders[M]//BEUTLER E, LICHTMAN M A, COLLER B S, et al.Williams hematology.New York:McGraw-Hill, 1995:616-650.
[7] JOUTOVSKY A, HADZI NESIC J, NARDI M A.HPLC retention time as a diagnostic tool for hemoglobin variant and hemoglobinopathies:A study of 60000 samples in a clinical diagnosticiaboratory[J].Clin Chem, 2004, 50:1736-1747.
[8] 周玉球, 李文典, 徐湘民.用于血红蛋白病遗传筛查的实验室诊断技术[J].国际遗传学杂志, 2008, 31(1):17-22.
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