Combined detection of HCV antibody and HCV RNA in blood donation
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摘要: 目的:探讨丙型肝炎病毒抗体与核酸检测联合应用在血液筛查中的意义。方法:采用2种酶联免疫吸附(ELISA)试剂对2010-06-12在常州地区采集的无偿献血标本进行抗-HCV检测,对酶免阴性、单试剂阳性标本做核酸检测,并送卫生部临检中心进行确认。结果:24 546份血液标本中,2种ELISA试剂检测单阳性标本57例,经核酸筛查均呈阴性,但经卫生部临检中心确认有8例抗-HCV为阳性,17例抗-HCV为不确定。2种ELISA试剂检测均呈阴性的标本,经核酸筛查有39例呈阳性,经确认有2例HCV RNA阳性。对于抗-HCV确认结果为阳性及不确定的标本,2种试剂的S/CO最小值分别为0.62、1.07。结论:血液筛查检测HCV时,联合应用核酸检测和抗体检测可以进一步提高血液安全。Abstract: Objective:To explore the significance of combined detection of HCV antibody and HCV RNA in blood donation. Method:The blood samples collected from June 2010 to December 2010 in Changzhou were detected by two different anti-HCV ELISA kits. The both negative and single-positive anti-HCV samples were further tested for HCV RNA, and then confirmed by national center for clinical laboratory.Result:Totally 24 546 samples were collected. 57 samples were detected singly positive for HCV antibody, among which no positive HCV RNA was tested. However, 8 samples were confirmed positive anti-HCV, and 17 samples uncertain for anti-HIV. Among the samples detected both negative for HCV antibody, 39 samples were tested positively for HCV RNA, and 2 samples were confirmed positive. As for the samples confirmed positive and uncertain for HCV antibody, the minimum S/CO values of two kits were 0.62 and 1.07 respectively. Conclusion:Combined detection of HCV antibody and HCV RNA could further improve the blood safety.
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Key words:
- Hepatitis C virus /
- antibody /
- nucleic acid /
- blood screening
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