血液HBV、HCV和HIV核酸检测在临床输血中的应用

黄劲松; 刘世国; 张军霞; 彭黎; 李勤; 徐瑶; 岑千红

血液HBV、HCV和HIV核酸检测在临床输血中的应用

- 湖北省中山医院检验科(武汉, 430030)
基金项目:
2011-2012年度湖北省卫生厅采供血管理科研项目（No：CGX2010-5）

详细信息

通讯作者: 岑千红,E-mail:cenqianhong@163.com

中图分类号: R457.1

收稿日期: 2012-08-20

Application of NAT testing for HBV, HCV and HIV in clinical blood transfusion

- Department of Clinical Laboratory, Hubei Zhongshan Hospital Wuhan, 430030, China

More Information

Corresponding author: CEN Qianhong, E-mail: cenqianhong@163.com

Received Date: 20 August 2012

摘要

摘要: 目的：探讨采供血机构血液制品和综合医院输血常规检测病人同时进行病毒核酸筛查的必要性和可行性。方法：采集湖北省中山医院输血科2010-05-2011-05的武汉市血液中心来源血液制品3 527份，以及2012-01-2012-07的输血常规检测标本中各项血清学筛查均合格的2 815份血液标本作16人份混合血清样本病毒核酸扩增（NAT）检测，凡筛查不合格血样再作单人份检测。结果：HBVDNA阳性标本4例，HCVRNA和HIVRNA阳性标本未检出。结论：采用NAT技术同时筛查献血者和受血者，可以进一步有效降低输血安全风险，避免因输血传播疾病造成的医疗纠纷。
- 病毒核酸扩增技术 /
- 输血安全 /
- 输血前检查
Abstract: Objective: To study the necessity and feasibility of NAT testing for HBV,HCV and HIV in clinical blood transfusion.Method: 3 527 samples from Wuhan Blood Center from 2010 May to 2011 May and 2 815 samples from Department of Clinical Laboratory,Hubei Zhongshan Hospital from 2012 January to 2012 July were collected and detected by nucleic acid amplification (NAT) testing for pools of 16 serologically negative samples,any pools positive in NAT would undergo NAT again individually.Result: HBVDNA positive specimens were found from 4 patients,HCVRNA and HIVRNA positive samples were not detected.Conclusion: Screening of blood donors and blood recipients using the technology of NAT could further reduce blood transfusion safety risk and avoid medical disputes caused by transfusion transmitted diseases.
- nucleic acid amplification testing /
- blood transfusion safety /
- examination before blood transfusion

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参考文献(11)

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