人巨细胞病毒AD169株UL115基因编码gL蛋白序列分析及B细胞表位预测

魏中南; 胡洪波; 彭巧英

人巨细胞病毒AD169株UL115基因编码gL蛋白序列分析及B细胞表位预测

- 1 湖北省妇幼保健院检验科(武汉, 430070);
- 2 湖北省妇幼保健院新生儿科

详细信息

通讯作者: 胡洪波,E-mail:dingguang666@hotmail.com

中图分类号: R552

收稿日期: 2012-01-19

Study on B-cell epitopes of glycoprotein L expressed by human cytomegalovirus UL115 gene of AD169 strain

- 1. Department of Clinical Laboratory, Hubei Maternal and Child Health Hospital, Wuhan, 430070, China;
- 2. Department of Neonatology, Hubei Maternal and Child Health Hospital

More Information

Corresponding author: HU Hongbo, E-mail: dingguang666@hotmail.com

Received Date: 19 January 2012

摘要

摘要: 目的:分析人巨细胞病毒(HCMV)AD169株UL115基因序列,预测UL115基因编码的gL蛋白B细胞优势表位。方法:基于UL115基因编码的gL蛋白的氨基酸序列,结合亲水性参数、可及性参数、抗原性参数、柔韧性参数及二级结构方案对HCMV gL蛋白的B细胞表位进行预测,参照已建立的预测方法综合评价B细胞优势表位。结果:①UL115核酸变异集中在序列的N端,大部分是同义突变,氨基酸序列高度保守;②HCMV gL蛋白 B细胞表位可能位于编码蛋白N段197~205、253~261位。结论:多参数预测gL蛋白的B细胞优势表位,为进一步研究蛋白特征、制备单克隆抗体及表位疫苗提供依据。
- 人巨细胞病毒 /
- AD169株 /
- UL115基因 /
- gL蛋白 /
- B细胞表位
Abstract: Objective:To analyze the sequence of genome of cytomegalovirus UL115 and predict the B cell epitopes of the gL protein expressed by cytomegalovirus UL115.Method:The hydrophilicity,accessibility,antigenicity and flexibility index were used to predict the potential B cell epitopes of gL protein based on gL genome sequence.Result:① Most amino acid sequence of gL was highly conserved although several strains had variation.Those mutations focused on the N end of U115,but most of them were sense mutation.② The B cell epitopes of gL protein generated by combined application were predicted at gL protein N-terminal 197-205,253-261.Conclusion:The B-epitopes of gL protein was predicted successfully,which established the basis of the characterization of the protein,development of epitopes based vaccine,and preparation of monoclonal antibody against fusion protein.
- human cytomegalovirus /
- AD169 stvain /
- UL115 gene /
- envelope glycoprotein L /
- B cell epitopes

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参考文献(6)

[1]	VANARSDALL A L,CHASE M C,JOHNSON D C.Human cytomegalovirus glycoprotein go complexes with gH/gL,promoting interference with viral entry into human fibroblasts but not entry into epithelial cells[J].J Virol,2011,85:11638-11645.
[2]	BOWMAN J J,LACAYO J C,BURBELO P,et al.Rhesus and human cytomegalovirus glycoprotein L are required for infection and cell-to-cell spread of virus but cannot complement each other[J].J Virol,2011,85:2089-2099.
[3]	KOLASKAR A S,TONGAONKAR P C.A semi-empirical method forprediction of antigenic determinants on protein antigens[J].FEBS Lett,1990,276:172-174.
[4]	KARPLUS P A,SCHULZ G E.Prediction of chain flexibility in proteins[J].Naturwissenschaften,1985,72:212-213.
[5]	EMINI E A,HUGHES J V,PERLOW D S,et al.Induction of hepatitis a virus-neutralizing antibody by a virus-specific synthetic peptide[J].J Virol,1985,55:836-839.
[6]	吴玉章,朱锡华.一种病毒蛋白B细胞表位预测方法的建立[J].科学通报,1994,39(24):2275-2279.