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摘要: 目的：比较一步法与两步法抗-HCV ELISA检测试剂的特异性及灵敏度。方法：分别采用两个不同厂家的一步法及两步法ELISA试剂，对本中心标本进行试验，对比分析两种试剂的血液报废率及检测结果。结果：使用两步法ELISA检测试剂后，血液报废率相对于一步法检测试剂略有下降，可疑阳性标本数明显下降。但灰区和可疑弱阳性标本数两个厂家间有较大差异。不同的两步法检测试剂符合率仍有待提高。结论：两步法抗-HCV ELISA检测试剂优于一步法，但特异性及灵敏度仍有待加强。Abstract: Objective: To compare the specificity and sensitivity of the one step and two step anti-HCV ELISA reagents.Method: Specimens of the center were respectively detected using two different manufacturer of one-step and two-step ELISA reagents.The detection results and blood scrap rate were compared.Result: After using two-step ELISA detection reagent,the blood scrap rate fell slightly compared with one-step detection reagent,the suspicious positive specimens decreased obviously.But the gray area specimens and suspicious weakly positive samples had great difference between the two manufacturers.Two step detection reagents coincidence rate remained to be improved.Conclusion: Two-step anti-HCV ELISA detection reagents would be superior to the one step method,but the specificity and sensitivity remain to be strengthened.
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Key words:
- hepatitis C virus /
- one-step method /
- two-step method /
- ELISA
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