Abstract: Objective: To analyze retesting results and disqualification situation of the blood screening for RH D-individuals in voluntary blood donors in Nanjing,and explore the strategy to reduce waste of these rare blood resources.Method: The samples of voluntary blood donors in Nanjing from 2006-2011 were retested after initial rapid detection and the results were analyzed.Result: The D-blood proportion was decreasing year by year despite a rally in 2011,while the unqualified rate of the D-blood products was gradually increasing in recent years,which from 0.28% (in 2006) to 1.92% (in 2011).The main cause of disqualification was HBsAg and ALT.Among the unqualified group,the number of 200 ml blood donators was rising,with the unvariably dominating role of the 300 ml and 400 ml blood donators.Meanwhile,the ratio of the unfixed blood donors to the fixed ones was 19 to 7.The unqualified group distribution was mainly composed of married male young adults,most of whom were from the suburb of Nanjing.However,well educated population and external population tended to grow in number.Conclusion: Not only improving the quality of detection reagent and standardizing operative technique would be helpful to reduce waste of D-blood products,but also strengthening propaganda and recruitment work would be significant to promote sustainable development for rare blood donation cause,especially strengthening the recruitment of repeat donors and the donors who might return after temporary deferral.