再生障碍性贫血患者CX3CR1介导的T细胞趋化和黏附功能异常

侯晓燕; 王琳琳; 任娟; 李辉; 彭军

再生障碍性贫血患者CX3CR1介导的T细胞趋化和黏附功能异常

- 山东大学齐鲁医院血液科(济南, 250000)
基金项目:
山东省优秀中青年科研奖励基金（No：BS2009YY049）

详细信息

通讯作者: 彭军,E-mail:junpeng88@sina.com

中图分类号: R556

收稿日期: 2012-09-12

修回日期: 2012-11-13

CX3CR1-mediated chemotactic and adhesive dysfunction of T cells in aplastic anemia patients

- Department of Hematology, Qilu Hospital of Shandong University, Jinan, 250012, China

More Information

Corresponding author: PENG Jun, E-mail: junpeng88@sina.com

Received Date: 12 September 2012

Revised Date: 13 November 2012

摘要

摘要: 目的：探讨CX3CL1/CX3CR1对获得性再生障碍性贫血患者CD4⁺和CD8⁺T细胞迁移和黏附功能的影响。方法：17例重型再障患者和10例健康对照，应用MACS免疫磁珠分选法分选CD4⁺和CD8⁺T细胞，在自身骨髓血浆或CX3CR1的配体CX3CL1的作用下通过Transwell迁移实验比较患者和健康对照CD4⁺和CD8⁺ T细胞表面CX3CR1介导的的趋化活性以及阻断CX3CR1对其趋化活性的影响；采用细胞基质黏附实验比较患者和健康对照CX3CL1/CX3CR1介导的CD4⁺和CD8⁺T细胞的黏附能力。结果：再障患者骨髓CD4⁺和CD8⁺T细胞CX3CL1/CX3CR1介导的的迁移活性及黏附能力均高于正常对照组（P<0.05），尤以CD8⁺细胞更为显著（P<0.05）。结论：CX3CL1/CX3CR1的相互作用可能参与了T细胞介导的再障的发病机制，对CX3CR1的阻断有望成为治疗再障的新靶点。
- 再生障碍性贫血 /
- CX3CR1 /
- T淋巴细胞 /
- hCX3CL1类似物
Abstract: Objective: To investigate the effect of Fractalkine/CX3CR1 on CD4⁺ and CD8⁺ T lymphocytes migration and adhesion in patients with acquired aplastic anemia.Method: Seventeen patients with aplastic anemia(AA) and 10 healthy controls matched with age and gender were enrolled in this study.Immunomagnetic beads sorting was used to isolate CD4⁺ and CD8⁺ T lymphocytes from the bone marrow of AA patients and healthy controls.Transwell migration assay was used to compare CX3CR1-mediated chemotaxis of CD4⁺ and CD8⁺ T lymphocytes to autologous bone marrow plasma or CX3CR1 ligand and to analyze the effect of CX3CR1 blockage on the chemotaxis activity.Cell matrix adhesion assay was used to demonstrate CX3CL1/CX3CR1-mediated CD4⁺ and CD8⁺ T lymphocyte adhesion in SAA patients as compared with healthy controls.Result: CX3CR1-mediated chemotaxis and adhesion ability in bone marrow CD4⁺ and CD8⁺ T lymphocyte in AA patients was higher than healthy controls (P<0.05), especially in CD8⁺ T lymphocytes(P<0.05).Conclusion: The interaction of Fractalkine with CX3CR1 may be involved in the T cell mediated pathogenesis of aplastic anemia, suggesting the blockage of CX3CR1 as a promosing therapy target in aplastic anemia.
- aplastic anemia /
- CX3CR1 /
- T Cells /
- hCX3CR1 analog

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