Abstract: Objective: Study on the TIM4 expression levels of peripheral blood mononuclear cells in patients with eczema and rheumatoid arthritis. Method: The TIM4 positive cells proportion of the peripheral blood mononuclear were detected using the flow cytometry technique to in thirty healthy people, thirty patients with eczema, thirty patients with rheumatoid arthritis and thirty patients with elevated IgE. Eosinophils, monocytes, and lymphocytes accounted for the proportion of white blood cells were tested, and their correlation with TIM4 level were analyzed. Result: Compared with healthy controls, the TIM4 positive cell percentage of peripheral blood mononuclear cells in the patients with eczema and patients with RA were significantly increased. The difference had statistically significance (P<0.05), but the group of elevated IgE had no statistically difference (P>0.05).Compared with healthy controls, the proportion of eosinophils were significantly increased in the group of patients with eczema. The difference had statistically significance (P<0.05), but the patients with rheumatoid arthritis and patients of elevated IgE had no statistically difference (P>0.05). Each TIM4 and monocytes, TIM4 and lymphocyte were no significant correlation (P>0.05). In addition, the TIM4 proportion of the rheumatoid arthritis group was associated with the age of the patient (P>0.05). Other groups had no correlation. Conclusion: Compared to the healthy ones, TIM4 expression levels in patients with eczema and RA significantly increased. TIM4 would has relation with disease such as eczema and rheumatoid arthritis.