Duffy抗原基因多态性与结直肠癌易感性的关联分析

周世航; 梁晓华; 杨悦; 刘铭

doi:10.13201/j.issn.1004-2806-b.2016.02.006

Duffy抗原基因多态性与结直肠癌易感性的关联分析

- 1 大连市血液中心(辽宁大连, 116001);
- 2 大连医科大学检验医学院;
- 3 大连医科大学细胞生物学教研室

详细信息

通讯作者: 刘铭,E-mail:240702974@qq.com

中图分类号: R735.3

收稿日期: 2015-07-16

Association of Duffy antigen gene polymorphisms with the susceptibility of colorectal carcinoma

- 1 Dalian Blood Center, Dalian, 116001, China;
- 2 College of Medical Laboratory, Dalian Medical University;
- 3 Department of Cell Biology, Dalian Medical University

More Information

Corresponding author: LIU Ming, E-mail: 240702974@qq.com

Received Date: 16 July 2015

摘要

摘要: 目的: 探讨Duffy抗原基因rs863002和 rs3027012位点的单核苷酸多态性(SNP)与结直肠癌易感性的关系。方法: 收集100例结直肠癌患者和100例健康对照者的外周血样本,提取全血DNA;选择Duffy抗原基因2个具有代表性的标签SNP位点(rs863002和rs3027012),使用TaqMan-MGB探针Real-time PCR技术对样本进行基因分型。结果: rs863002位点的CC基因型频率在结直肠癌组和对照组分别为90.0%和88.0%,CT基因型频率分别为10.0%和12.0%,两组间差异无统计学意义(P>0.05)。rs863002位点的C等位基因频率在结直肠癌组和对照组中分别为95.0%和94.0%,T等位基因频率分别为5.0%和6.0%,两组间差异无统计学意义(P>0.05)。rs3027012位点的CC基因型频率在结直肠癌组和对照组分别为97.0%和98.0%,CT基因型频率分别为3.0%和2.0%,两组间差异无统计学意义(P>0.05)。rs3027012位点C等位基因频率在结直肠癌组和对照组中分别为98.5%和99.0%,T等位基因频率分别为1.5%和1.0%,两组间差异无统计学意义(P>0.05)。结论: 尚未发现Duffy抗原基因rs863002和 rs3027012位点SNP与结直肠癌易感性存在关联。
- Duffy抗原 /
- 结直肠癌 /
- 单核苷酸多态性 /
- 易感性
Abstract: Objective: To examine the association of single nucleotide polymorphisms (SNPs) (rs863002 and rs3027012) at Duffy antigen gene with the susceptibility of colorectal carcinoma. Method: Whole blood samples were collected from 100 individuals with colorectal carcinoma and 100 normal controls. The DNA of the whole blood samples was extracted and then the selected SNPs (rs863002 and rs3027012)at Duffy antigen gene were genotyped by real-time PCR technique with TaqMan-MGB probe. Result: In colorectal carcinoma group and control group, the frequencies of CC at rs863002 were 90.0% and 88.0% respectively and the frequencies of CT were 10.0% and 12.0% respectively. There were no statistically significant difference between the two groups (P>0.05). The frequencies of C were 95.0% and 94.0% respectively and the frequencies of T were 5.0% and 6.0% respectively. There were no statistically significant difference between the two groups (P>0.05). The frequencies of CC at the rs3027012 were 97.0% and 98.0% respectively and the frequencies of CT were respectively 3.0% and 2.0%. There were no statistically significant difference between the two groups (P>0.05). The frequencies of C were respectively 98.5% and 99.0%; the frequencies of T were respectively 1.5% and 1.0%. There were no statistically significant difference between the two groups (P>0.05). Conclusion: No association was found between the selected SNPs of Duffy antigen gene and the susceptibility of colorectal carcinoma.
- Duffy antigen /
- colorectal carcinoma /
- SNP /
- susceptibility

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