Pre and post-operative changes and its clinical significance of levels of TNF-α,NO,IL-1β and IL-6 in patients with colorectal cancer
-
摘要: 目的: 探索结直肠癌患者手术前后肿瘤坏死因子-α(TNF-α)、一氧化氮(NO)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)表达水平的变化及其临床意义。方法: 对75例试验组结直肠癌患者和对照组43例健康体检者分别采用硝酸还原法及双抗夹心ELISA法检测手术前及手术7 d后NO及TNF-α、IL-1β、IL-6表达水平,比较2组结果变化,同时对手术前后的检测指标作相关性分析。结果: 结直肠癌患者手术前血清中TNF-α、IL-1β、IL-6浓度明显高于正常对照组(P<0.05),而NO浓度明显低于正常对照组(P<0.05),手术治疗后7 d,TNF-α、IL-1β、IL-6血清含量明显降低(P<0.05),而NO含量恢复正常水平。手术前后TNF-α与IL-6、NO、IL-1β及NO与IL-6、IL-1β均无相关性,而IL-1β与IL-6之间呈明显的正相关。结论: 血清中TNF-α、NO、IL-1β、IL-6浓度与结直肠癌密切相关,可作为结直肠癌诊断及手术治疗新的参考指标。Abstract: Objective: To explore the pre and post-operative changes and its clinical significance of the levels of TNF-α, NO, IL-1β and IL-6 in the patients with colorectal cancer. Method: The levels of NO and TNF-α, IL-1β, IL-6 in 75 colorectal cancer patients and control group were detected by nitrate reduction and double-antibody sandwich ELISA, and the results were compared. Result: In preoperative colorectal cancer patients, the levels of serum TNF-α, IL-1β, IL-6 were significantly higher than those in controls (P<0.05), while the NO level was significantly lower (P<0.05). Seven days after operation, the levels of TNF-α, IL-1β, IL-6 were lower than those before (P<0.05), and the NO level returned to normal. Conclusion: TNF-α, NO, IL-1β and IL-6 might play important roles in colorectal cancer, and they may provide new reference for colorectal cancer early diagnosis.
-
Key words:
- colorectal cancer /
- TNF-α /
- NO /
- IL-1β /
- IL-6
-
-
[1] 田雅军,王立君.结直肠癌中血管内皮生长因于和一氧化氮表达及其临床意义[J].临床研究, 2013,10(2):46-50.
[2] 张利忠,周建平.结直肠癌的新辅助化疗[J].长治医学学报, 2014,28(2):157-160.
[3] 戚文娟,田雪娇.代谢组学在结直肠癌中的研究进展[J].医学与哲学, 2013, 34(6B):62-64.
[4] Askling J, Dickman PW, Karlen P,et al. Family history as a risk factor for colorectal cancer in inflammatory bowel disease[J]. Gastroenterology, 2011, 120:1356-1362.
[5] Ankathatti Munegowda M, Deng Y, Mulligan SJ, et al. Th17 and Th17-stimulated CD8+T cells play a distinct role in Th17-induced preventive and therapeutic antitumor immunity[J]. Cancer Immunol Immunother, 2011, 60:1473-84.
[6] 王振宁.结直肠癌TNM分期的现状及发展方向[J].中华结直肠疾病电子杂志,2015,(1):5-7.
[7] 陆君阳,于健春.肥胖、炎症与结直肠癌的关系研究进展[J].中华医学杂志,2014,(14):1117-1119.
[8] 王建升,帖彦清,张立志,等.结直肠癌患者外周血Tc17细胞在肿瘤进展中的变化[J].中国免疫学杂志,2014,(6):817-820.
[9] Hsu CP, Chung YC. Influence of interleukin-6 on the invasiveness of human colorectal carcinoma[J]. Anticancer Res, 2012, 26:4607-4614.
[10] 王建升,张立志,帖彦清,等.结直肠息肉、结直肠腺瘤和结直肠癌患者外周血中Th17和Tc17细胞的变化[J].天津医药,2014,(4):312-314.
[11] 李云霄,陈涛.一氧化氮与肿瘤的研究进展[J].癌变·畸变·突变,2014,(4):317-320.
-
计量
- 文章访问数: 545
- PDF下载数: 141
- 施引文献: 0
下载: