Role of fructose-1,6-diphosphate on coagulation function and inflammatory cytokines in rats with sepsis
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摘要: 目的:探讨1,6-二磷酸果糖(FDP)对脓毒症大鼠凝血功能及炎症因子的影响。方法:依据随机数字表,60只健康成年雄性大鼠分成6组,分别为生理盐水对照组(NSc)、CLP组(经典盲肠结扎穿孔术)、FDP组(FDP0.33、FDP1.65、FDP3.30)以及FDP对照组(FDPc)。利用经典盲肠结扎穿孔术(cecal ligation and puncture,CLP)构建脓毒症大鼠模型。生理盐水对照组(NSc)行假手术加生理盐水输注。CLP组行CLP术加生理盐水输注。FDP组采用CLP加不同浓度的FDP输注;根据输入浓度的不同,分为3组,分别输注FDP浓度0.33g/kg(FDP0.33),1.65g/kg(FDP1.65),3.30g/kg(FDP3.30)。FDP对照组(FDPc)行假手术加FDP输入,浓度为3.30g/kg。各组液体输入时间为CLP术后3h。术后6h及12h分别采血测各组大鼠血浆肿瘤坏死因子α(TNF-α)、白介素6(IL-6)及凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、纤维蛋白原(Fib)及血栓弹力图(TEG)等。比较各组大鼠各时间点生存率,时间点分别为6h、12h、24h及48h。结果:同生理盐水对照组(NSc)相比,CLP组及FDP组(FDP0.33、FDP1.65、FDP3.30)术后6h、12h大鼠血浆TNF-α、IL-6显著升高(P<0.01)。FDP各组(FDP0.33、FDP1.65、FDP3.30)TNF-α、IL-6浓度显著低于CLP组(P<0.05)。术后6h及12hFDP1.65与FDP3.30组中PT、APTT、Fib显著低于CLP组(P<0.05),FDP0.33组与CLP组差异无统计学意义(P>0.05)。术后6h及12hFDP各组(FDP0.33、FDP1.65、FDP3.30)R、K值显著低于CLP组(P<0.05)。术后6h及12hFDP1.65组及FDP3.30组MA值显著低于CLP组(P<0.05)。各组α及CI值在各时间点差异无统计学意义(P<0.05)。各组大鼠48h生存率差异无统计学意义(P>0.05)。结论:FDP能抑制脓毒症大鼠炎症反应并在早期能改善脓毒症大鼠凝血功能。Abstract: Objective:To investigate the role of fructose-1,6-diphosphate on coagulation function and inflammatory cytokines in rats with sepsis.Method:A total of 60 healthy adult male rats were randomly divided into 6 groups.They were Saline control group(NSc),CLP group,FDP group (FDP0.33,FDP1.65,FDP3.30) and FDP control group (FDPc),respectively.The animal mode of sepsis in rats was made by using classical cecum ligation and puncture (CLP).NSc group underwent sham operation and saline injection.CLP group underwent CLP operation and saline injection.FDP group (FDP0.33,FDP1.65,FDP3.30) underwent CLP operation and FDP injection.FDP group was divided into 3groups by the different concentration,including the injection of FDP concentration with 0.33g/kg(FDP0.33),1.65g/kg(FDP1.65)and 3.30g/kg(FDP3.30).FDPc group underwent sham operation and FDP injection with 3.30g/kg.The time of injection in each group was 3 hours after CLP operation.TNF-α,IL-6,PT,APTT,Fib and Thrombelastogram(TEG)were measured at the 6hours and 12 hours after operation in each group respectively.The survival rates in each group at the time of 6 hours,12 hours,24 hours,48 hours and 72 hours were compared.Result:Compared with NSc group,the concentrations of TNF-α and IL-6 were increased significantly (P<0.01) in CLP group and FDP group(FDP0.33,FDP1.65,FDP3.30) at the time of 6 hours and 12 hours after operation.The concentrations of TNF-α and IL-6 in FDP group(FDP0.33,FDP1.65,FDP3.30) were less than those in CLP group(P<0.05).The concentrations of PT,APTT and Fib in FDP1.65 group and FDP3.30 group were less than those in CLP group at the time of 6 hours and 12 hours(P<0.05).There was no significance between FDP0.33 group and CLP group(P>0.05).At the time of 6 hours and 12 hours after operation,the values of R and K in FDP group(FDP0.33,FDP1.65,FDP3.30)were less than those in CLP group(P<0.05).At the time of 6 hours and 12 hours after operation,the values of MA in FDP1.65 group and FDP3.30 group were less than that in CLP group(P<0.05).The values ofαand CI in each group at each time point had no significance(P>0.05).The survival rate had no significance between each group at the time of 48 hours.Conclusion:FDP could inhibit the inflammatory reaction in rats with sepsis and improve the coagulation function in the early stage of sepsis.
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Key words:
- fructose-1 /
- 6-diphosphate /
- sepsis /
- inflammatory cytokines /
- coagulation function
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