Abstract: Objective:To analyze the causes of red blood cell transfusion and injection effect.Method:Direct antiglobulin test,antibody screening and blood routine detection and antibody detection of 11 cases of poor effectiveness of red blood cell transfusion were done before transfusion,blood transfusion after 3 days,7 days and 12 days respectively.Result:The results of 11 cases of the red blood cell transfusion poor effect before transfusion were all negative,at the 7th day after blood transfusion the results of hemoglobin and red blood cell count began to appear increased then decreased,the direct antiglobulin test results were positive or suspicious,at the 12 th day antibody screening results of all cases were positive or suspicious.Conclusion:The reasons of 11 cases of red blood cell transfusion poor effect were in line with the characteristics of "recall response".This suggested that for the patients with history of blood transfusion or multiple pregnancy history,even if the antibody screening test results were negative,it also should consider the possibility of "recall response".So it is necessary for patients to receive blood transfusion with Rh antigen(D/C/c/E/e)matched,in order to ensure safe and effective transfusion.