Rh serological phenotype distribution and irregular antibody detection of Rh negative blood donors in Shijiazhuang area
-
摘要: 目的:研究石家庄地区献血者RhD阴性血清学表型分布和不规则抗体检出情况。方法:对2015-01-2015-12石家庄地区无偿献血者标本,进行RhD阴性血筛查,确认血清学表型鉴定和不规则抗体筛选。结果:172 515例献血者标本中筛查出892例RhD阴性(0.52%),经确认为831例RhD阴性(0.48%),D变异型61例(6.84%),表型鉴定结果为ccdee 514例(57.62%)、Ccdee 222例(24.89%)、ccdEe 50例(5.61%)、CCdee 34例(3.81%)、CcdEe 20例(2.24%)、CCdEe 2例(0.22%);筛查出的892例RhD阴性标本中检出不规则抗体26例(2.8%)。结论:对献血者进行Rh阴性血筛查、确认,血清学表型鉴定和不规则抗体筛选对保障安全用血具有重要意义。Abstract: Objective:To study Rh serological phenotype distribution and irregular antibody detection of RhD negative blood donors in Shijiazhuang area.Method:RhD negative blood was screened and comfirmed,Rh phebotype was indentified,irregular antibody was detected of unpaid donors in Shijiazhuang area from January 2015 to December 2015.Result:A total of 892 RhD negative (0.52%)were screened in 172 515 donors,among which 831 RhD negative were comfirmed (0.48%),61 samples were D variants,phenotype indentification were ccdee 514(57.62%),Ccdee 222(24.89%),ccdEe 50(5.61%),CCdee(34 3.81%),CcdEe 20(2.24%) and CCdEe 2(0.22%).Twenty-six(2.8%)irregular antibody were screened in 892 RhD negative.Conclusion:It is very important to ensure blood safety by screening and comfirming RhD negative blood,indentifying Rh phebotype and dectecting irregular antibody.
-
Key words:
- RhD negative blood /
- phenotype /
- irregular antibody
-
-
[1] 马红丽,郑备战,吴文静.洛阳地区初筛RhD阴性献血者Rh血型抗原分布情况[J].中国输血杂志,2016,29(4):408-409.
[2] 周国平,谢云峥,向东,等.上海市Rh阴性献血者D变异型与不规则抗体调查[J].临床检验杂志,2009,27(3):233-234.
[3] 朱自严,译.人类血型[M].北京:科学出版社,2007:246-259.
[4] 段福才,曹燕飞,李建华,等.Rh弱D和及Del不规则抗体的检测及意义探讨[J].临床血液学杂志,2013,26(6):420-421.
[5] 郑望春,杨转笑,容莹,等.深圳地区Rh阴性无偿献血者血清学表型与不规则抗体调查[J].国际检验医学杂志,2013,34(10):1256-1270.
[6] 车进,杨亚玲,李美霖.北京地区Rh(D)阴性献血者分型及不规则抗体筛查[J].山西医药杂志,2015,44(7):740-742.
[7] 蔡隽.金华市RhD阴性献血者Rh血型表型库的建立及应用[J].中国输血杂志,2013,26(9):790-791.
[8] Sambrook J,Ruaaell DW.Molecular cloning:a laboratory manual[M].3rd ed.New York:Cold Spring Harbor Laboratory Press,2001:16-34.
[9] 王连友,毕星秀,曹红荣,等.徐州地区RhD阴性献血者血清学表型和不规则抗体的研究[J].临床血液学杂志,2015,28(2):142-144.
-
计量
- 文章访问数: 280
- PDF下载数: 191
- 施引文献: 0
下载: