急性白血病引起的贫血患者血型鉴定、抗体筛查及交叉配血的检测

杨利拥; 桂木梅; 廖兵; 张霞

doi:10.13201/j.issn.1004-2806-b.2019.04.007

急性白血病引起的贫血患者血型鉴定、抗体筛查及交叉配血的检测

- 1. 重庆市第九人民医院检验科(重庆, 400700);
- 2. 重庆市长寿区人民医院输血科

详细信息

通讯作者: 廖兵,E-mail:ylypop@163.com

中图分类号: R733.71;R556

收稿日期: 2018-12-18

Blood group identification, antibody screening and cross-matching detection methods and results analysis of anemia caused by acute leukemia

- 1. Department of Clinical Laboratory, Chongqing Ninth People's Hospital, Chongqing, 400700, China;
- 2. Department of Blood Transfusion, Changshou District People's Hospital

More Information

Corresponding author: LIAO Bing, E-mail: ylypop@163.com

Received Date: 18 December 2018

摘要

摘要: 目的：研究急性白血病引起的贫血患者血型鉴定、抗体筛查及交叉配血的检测方法。方法：选取2015-05-2018-05收治的200例急性白血病引起的贫血患者，做血型鉴定、抗体筛查及交叉配血输血前3项检查，记录自身抗体分型、ABO和Rh血型鉴定结果，温型、冷型及混合型抗体的筛选和鉴定，交叉配血结果。结果：200例急性白血病引起的贫血患者中，温抗体、冷抗体、混合型抗体分别为128例（64.00%）、50例（25.00%）、22例（11.00%）。血型鉴定中反定型存在48例抗体干扰，正定型89例抗体干扰。交叉配血的50例冷抗体患者中，有特异性自身抗体17例（34.00%），PEG-IAT法配血全部成功；无特异性自身抗体33例（66.00%），PEG-IAT法配血成功30例（90.90%），Coombs法符合者3例（9.10%）。混合型抗体阳性患者22例中，自身抗体+同种抗体4例（18.18%），Coombs法配血全部成功；无特异性自身抗体18例（81.82%），其中PEG-IAT法配血成功15例（83.33%），Coombs法符合者3例（16.67%）。温抗体阳性患者128例中，无特异性自身抗体29例（22.66%），其中PEG-IAT法配血成功21例（72.41%），Coombs法符合者8例（27.59%）；特异性自身抗体21例（16.41%），PEG-IAT法配血全部成功；自身抗体+同种抗体78例（60.94%），其中PEG-IAT法配血成功2例（2.56%），Coombs法符合者76例（97.44%）。结论：急性白血病引起的贫血患者在输血时所做的输血前3项检查中，选择合适的检测方法能降低检测成本，提高检验效率及安全性，降低输血时发生不良反应的风险。
- 急性白血病 /
- 贫血 /
- 血型鉴定 /
- 抗体筛查 /
- 交叉配血
Abstract: Objective: To study the blood group identification, antibody screening and cross-matching method for patients with anemia caused by acute leukemia.Method: A total of 200 patients with anemia caused by acute leukemia from May 2015 to May, 2018 were selected for blood group identification, antibody screening and cross-matching blood transfusion.The results of autoantibody typing, ABO and Rh blood group identification, screening and identification of warm, cold and mixed antibodies and cross-matching were recorded.Result: Of the 200 patients with anemia caused by acute leukemia, the warm, cold and mixed antibody were 128 cases(64.00%), 50 cases(25.00%) and 22 cases(11.00%), respectively.There were 48 cases of antibody interference in the anti-type of blood type identification, and 89 cases of antibody interference in positive type.Among the 50 cold antibody patients with cross matching, there were 17 cases(34.00%) with specific autoantibodies, all of which were successfully matched by PEG-IAT method;33 cases(66.00%) without specific autoantibodies, PEG-IAT method with blood 30 cases were successful(90.90%) and 3 cases(9.10%) were consistent with the Coombs method.Among the 22 patients with mixed antibody positive, autoantibody+allogeneic antibody in 4 cases(18.18%), Coombs method was all successful;18 cases(81.82%) had no specific autoantibodies, and 15 cases(83.33%) were successfully matched by PEG-IAT method Coombs method was consistent with 3 cases(16.67%).Among the 128 patients with positive warm antibody, there were 29 cases(22.66%) without specific autoantibodies, 21 cases(72.41%) with PEG-IAT method, and 8 cases(27.59%) with Coombs method;specific autoantibodies in 21 cases(16.41%), the PEG-IAT method was successfully matched with blood;autoantibody + alloantibodies in 78 cases(60.94%), PEG-IAT method in 2 cases(2.56%), and Coombs method in 76 cases(97.44%).Conclusion: In the first three blood transfusion tests of patients with anemia caused by acute leukemia, choosing the appropriate test method can reduce the cost of testing, improve the efficiency and safety of testing, and reduce the risk of adverse reactions during transfusion.
- acute leukemia /
- anemia /
- blood type identification /
- antibody screening /
- cross matching

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