128例RhD阴性献血者DEL表型筛查与基因型分析

张剑; 项杰; 饶神宗; 张敏; 鲁学良; 向长港

doi:10.13201/j.issn.1004-2806-b.2019.10.013

128例RhD阴性献血者DEL表型筛查与基因型分析

- 1. 武汉市金银潭医院检验科(武汉, 430023);
- 2. 华中科技大学同济医学院附属协和医院输血科;
- 3. 华中科技大学同济医学院附属协和江南医院检验科

详细信息

通讯作者: 饶神宗,E-mail:1833703604@qq.com

中图分类号: R457.1

收稿日期: 2019-08-20

Del phenotypic screening and genotype analysis in 128 RhD negative blood donors

- 1. Department of Laboratory, Wuhan Jingyingtan Hospital, Wuhan, 430023, China;
- 2. Department of Transfusion, Union Hospital HUST;
- 3. Department of Laboratory, Union Jiang nan Hospital HUST

More Information

Corresponding author: RAO Shenzong, E-mail: 1833703604@qq.com

Received Date: 20 August 2019

摘要

摘要: 目的:分析武汉地区RhD阴性献血者中DEL表型的分布及RhD基因情况。方法:血清学直接凝集法检测D、C、c、E、e抗原,间接抗人球蛋白试验(IAT)确认RhD阴性血型,对IAT确认为RhD抗原阴性的样本采用吸收放散方法从中筛选DEL型;应用PCR-SSP技术检测DEL献血者RhD基因型。结果:血型血清学试验初筛为RhD阴性的128例献血者标本中,IAT试验检出阳性9例,经鉴定为D变异型;剩余119例样本中吸收放散结果阳性31例,确定为DEL型。119例RhD阴性献血者中68例ccee,39例Ccee,5例CCee,5例ccEe和2例CcEe。128例样本检出DEL型31例,占比为24.22%,表型分布为Ccee 23例,CCee 5例,ccEe 2例,CcEe 1例;送检的DEL型标本第9外显子均有1227G＞A突变,其中杂合子24例(DEL/d),纯合子7例(DEL/DEL)。结论:武汉地区DEL型献血者表型分布与其他地区相似,RhD 1227A是武汉地区DEL表型个体的重要遗传标记。
- RhD /
- 间接抗人球蛋白试验 /
- 基因 /
- PCR-SSP
Abstract: Objective:To analyze the distribution of DEL phenotype and RhD gene in RhD negative blood donors in Wuhan.Method:D,C,c,E and e antigens were detected by regular serological agglutination assay,RhD negative blood group was confirmed by indirect anti-human globulin test(IAT),The DEL type was detected by adsorption-elution assay for the samples confirmed by IAT to be negative for RhD antigen.The PCR-SSP technique was used to detect the DEL donors.Result:Among 128 blood donors whose RhD serological test was negative,9 were positive by IAT test and identified as D variant.31 of the remaining 119 samples were positive by adsorption-elution assay,and were identified as DEL type.Among 119 RhD negative donors,68 were ccee,39 were Ccee,5 were CCee,5 were ccEe and 2 were CcEe.Among 128 samples,31(24.22%)were found to be DEL type,with 23 cases of Ccee,5 cases of CCee,2 cases of ccEe and 1 case of CcEe.There were 1227G＞A mutations in exon 9 of DLE type specimens,including 24 heterozygotes(DEL/d)and 7 homozygotes(DEL/DEL).Conclusion:The phenotypic distribution of DEL blood donors in Wuhan is similar to that in other regions.RhD 1227A is an important genetic marker for DEL phenotypic individuals in Wuhan.
- RhD /
- indirect antiglobulin test /
- gene /
- PCR-SSP

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