1例ABO正反定不符的血型分子生物学鉴定

康丹; 郝一文

doi:10.13201/j.issn.1004-2806-b.2019.10.023

1例ABO正反定不符的血型分子生物学鉴定

康丹,
郝一文^,

- 中国医科大学附属第一医院输血科(沈阳, 110005)

详细信息

通讯作者: 郝一文,E-mail:hyw82666@163.com

中图分类号: R457.1

收稿日期: 2019-02-25

A case report of ABO positive-negative inconsistency in blood group molecular biology identification

KANG Dan,
HAO Yiwen^,

More Information

Corresponding author: HAO Yiwen, E-mail: hyw82666@163.com

Received Date: 25 February 2019

摘要

- 正反定不符 /
- 血型分子生物学 /
- 鉴定报告
Abstract: The practical value of combined use of serological methods and molecular biological methods in the identification of difficult blood type was explored through the identification of 1 case of ABO subtype blood group.The ABO blood group antigen of erythrocyte was determined by conventional blood serological detection method.The preliminary classification and sequencing of ABO blood group genes were performed using PCR-SSP.The haplotypes of DNA samples were sequenced by single nucleotide sequencing.Serological results showed that the specimens were positive for O type,reverse type for A type,serological method was unable to determine the blood type of patients;PCR-SSP results showed that the genotype was AxO1,AxO4/O2 type;direct sequencing and haplotype sequencing showed that the genotype of the specimen was Ael02.What did not match the serological positive and negative stereotypes was Ael02.Difficult blood group identification sometimes may require a combination of serological and molecular biological methods to accurately stereotype
- positive-negative inconsistency /
- blood group molecular biology /
- identification

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