毛细管超速离心法在临床输血中的应用

潘英芳; 宋永红; 赵爱平; 李京; 吕红娟; 周娜; 徐群

doi:10.13201/j.issn.1004-2806-b.2020.06.008

毛细管超速离心法在临床输血中的应用

- 山东省血液中心血型参比研究室(济南, 250012)

详细信息

通讯作者: 徐群,E-mail:xuqun88@126.com

中图分类号: R457.1

收稿日期: 2019-11-15

Application of capillary ultracentrifugation in clinical blood transfusion

- Blood Center of Shandong Province, Shandong, 250012, China

More Information

Corresponding author: XU Qun, E-mail: xuqun88@126.com

Received Date: 15 November 2019

摘要

摘要: 目的:利用毛细管超速离心技术鉴定新旧红细胞血型抗原,以便临床血型鉴定、抗体筛查,进而保证临床合理配血,确保输血安全。方法:对2015-01—2018-12多次输血交叉配血不合及疑难血型等21例患者进行分类,运用毛细管超速离心方法,分离新旧红细胞或输注红细胞,进行红细胞ABO血型抗原鉴定,Rh系统或及其他系统血型抗原鉴定,直接抗人球蛋白试验鉴定,明确鉴定血型及相应红细胞血型抗体。结果:经毛细管超速离心后,基本明确了2例患者血型,19例患者不规则抗体的特性,同时提示患者的直接抗人球试验阳性多为溶血性输血反应引起。结论:运用毛细管超速离心方法能够完善疑难血型鉴定,红细胞血型抗体的鉴定,有利于保障临床输血安全。
- 疑难血型 /
- 输血反应 /
- 毛细管超速离心技术 /
- 交叉配血 /
- 不规则抗体
Abstract: Objective:To identify new and old red blood cell antigens using capillary ultracentrifugation in order to perform clinical antibody screening and ensure rational blood matching and blood transfusion safety. Method:Twenty-one cases including unmatched blood type by cross-matching method and complicated blood type from January,2015 to December,2018 in the department were classified.The old and new red blood cells and transfusion blood cells were separated by capillary ultracentrifugation,followed by the identification of ABO,Rh or other blood antigen,direct antiglobulin test,blood type and corresponding red blood cell type antibodies. Result:After capillary ultracentrifugation, the blood groups of 2 patients and the characteristics of irregular antibodies in 19 patients were identified, and it was suggested that the positive results of direct antiglobulin test were mostly caused by hemolytic transfusion reaction. Conclusion:Capillary ultracentrifugation could improve the identification of complicated blood types and RBC antibodies,which was helpful to the safety of clinical blood transfusion.
- complicated blood type /
- transfusion reaction /
- capillary ultracentrifugation /
- cross-matching /
- irregular antibody

HTML全文

参考文献(11)

[1]	中华人民共和国卫生部.中国输血技术操作规程[M].天津科学技术出版社,1997:62-64.
[2]	中华人民共和国卫生部医政司.全国临床检验操作规程(精)[M].3版.东南大学出版社,2006:246-270.
[3]	姚凤兰,任芙蓉,王卓妍,等.超速离心浓缩对提高血液NAT筛查不确定标本鉴别率的临床研究[J].北京医学,2009,31(11):687-690.
[4]	靖春旭,赵菲.ABO血型鉴2例混合视野结果分析[J].中国现代药物应用,2015,9(22):173-174.
[5]	Sharpe C,Lane D,Cote J,et al.Mixed field reactions in ABO and Rh typing chimerism likely resulting from twin haematopoiesis[J].Blood Transfus,2014,12:608-610.
[6]	吕晓燕.毛细管超速离心法在输血后患者Rh血型鉴定中的应用分析[J].临床医药文献电子杂志,2019,6(50):153.
[8]	Cheng CK,Lee CK,Lin CK.Clinically significant red blood cell antibodies in chronically transfused patients:a survey of Chinese thalassemia major patients and literature review[J].Transfusion,2012,52:2220-2224.
[9]	Ngoma AM,Mutombo PB,Ikeda K,et al.Red blood cell alloimmunization in transfused patients in sub-SaharanAfrica:A systematic review and meta-analysis[J].TransfusApher Sci,2016,54:296-302.
[10]	Poornima AP,Fazal S,Shaiji PS,et al.Red Blood Cell Alloimmunization in Multitransfused Pediatric Population in a Tertiary Care Hospital[J].Indian J Pediatr 2019,86:245-249.
[11]	Hendrickson JE,Tormey CA,Shaz BH.Red blood cell alloimmunization mitigation strategies[J].Transfus Med Rev,2014,28:137-144.
[12]	Shao CP,Zhao CJ,Wu CL,et al.Rh-Matched Transfusion through Molecular Typing for β-Thalassemia Patients Is Required and Feasible in Chinese[J].Transfus Med Hemother,2018,45:252-257.