心肌梗死患者凝血因子Ⅴ基因多态性和APCR及Hcy的关系

高丽霞; 吴克雄; 胡军; 东传令; 王学锋; 丁秋兰; 戴箐; 张学平; 王云霞

doi:10.13201/j.issn.1004-2806.2012.02.010

心肌梗死患者凝血因子Ⅴ基因多态性和APCR及Hcy的关系

- 1 克拉玛依市中心医院血液肿瘤科(新疆克拉玛依, 834000);
- 2 上海交通大学附属瑞金医院输血科;
- 3 新疆克拉玛依市中心医院检验科;
- 4 新疆乌鲁木齐市疾病预防控制中心

详细信息

通讯作者: 高丽霞,E-mail:gaolixia2006m@163.com

中图分类号: R542.2

收稿日期: 2011-04-26

修回日期: 2011-10-17

Analysis the relationship of Factor V,APCR,and Hcy in acute myocardial infarction patients

- 1. Department of Hematology and Oncology, Karamay Central Hospital, Karamay, Xinjiang, 834000, China;
- 2. The Blood Transfusion Affiliated Ruijin Hospital, Shanghai Jiaotong University;
- 3. Laboratory Karamay Central Hospital;
- 4. Xinjiang Urumqi Center for Disease Control and Prevention

More Information

Corresponding author: GAO Lixia, E-mail: gaolixia2006m@163.com

Received Date: 26 April 2011

Revised Date: 17 October 2011

摘要

摘要: 目的:研究心肌梗死患者活化蛋白C 抵抗(APCR)和凝血因子Ⅴ基因3种多态性及高同型半胱氨酸血症(Hcy)的关系。方法:心肌梗死患者和正常对照血浆中APCR检测采用APTT为基础的凝固法,Hcy检测采用微循环酶法,并用限制性内切酶片段多态性方法测定FⅤ G1691-A、G1091-C、A1090-G等3种基因多态性的发生情况。结果:心肌梗死患者APCR阳性占16.25％,Hcy阳性占57.12％,1例维吾尔族患者患者3种基因多态性阳性。结论:心肌梗死患者Hcy阳性率明显高于正常对照。Hcy可能是引起心肌梗死患者脑血栓形成的重要原因之一。心肌梗死患者存在APCR现象,维吾尔族人可能和凝血因子Ⅴ基因3种多态性有关。
- 心肌梗死 /
- 高同型半胱氨酸血症 /
- 活化蛋白C抵抗 /
- 凝血因子Ⅴ /
- 基因多态性
Abstract: Objective:To investigate the relationship of gene polymorphism of coagulation factor Ⅴ with activated protein C-resistance(APCR)and Hyperhomocysteinemi(Hcy)in acute myocardial infarction patients. Method:APCR,Hcy,and FⅤ G1691-A,G1091-C,A1090-G gene polymorphism were assayed in 80 acute myocardial infarction patients and 100 normal subjects. Result:Positive rate of APCR and Hcy in patients with acute myocardial infarction was 16.25％, 57.12％ respectively.There was one patients having 3 types of FⅤ gene mutation.Conclusion:There were especially high positive Hcy rate and Hcy may be one of the important causes of thrombosis in patient with acute myocardial infarction. APCR phenomenon existed in acute myocardial infarction patients of Uyghur,it may have relation with factor Ⅴ gene mutation.
- myocardial infarction /
- activated protein c-resistance /
- hyperhomocysteinemi /
- factor V /
- genepolymorphism

HTML全文

参考文献(5)

[1]	BERTINA R M,KOELENLAN B P,KOSTER T,et al.Muation in blood coaulation factor sociated with resistce to activated protein C[J].Nature,1994,369:64-67.
[2]	COOPER D N,CHEN J M,BALL E V,et al.Genes,mutations,and human inherited disease at the dawn of the age of personalized genomics[J].Hum Mutat,2010,31:631-55.
[3]	MIDDENDORF K,GOHRING P,HUEHNS T Y,et al.Prevalence of resistance against activated protein C resulting from factor V Leiden is significantly increased in myocardial infarction:investigation of 507 patients with myocardial infarction[J].Med Clinic I,2004,147:897-904.
[4]	OZKAN Y,OZKAN E,SIMSEK B.Plasma total homocysteine and cysteine levels cardiovascular risk factors in coronary heart disease[J].Int Cardiol,2002,82:267-277.
[5]	TWATARGIL A,SADAN G,KARASU E.Homoeysteine-induced changes in vascular reactivity of guinea-pig pulmonary arteriesrole of the oxidative stress and poly(ADP ribose)polymerase activation[J].Pulm Pharmacol Ther,2007,20:265-272.