Correlation between expression level of human epididymal protein 4 and disease in perimenopausalwomen with chronic kidney disease
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摘要: 目的:探讨人附睾分泌蛋白4(HE4)在绝经前后女性慢性肾脏疾病(CKD)患者中的表达水平及其与疾病分期和肾小球滤过率(eGFR)的相关性。方法:选取2017年1月—2019年6月收治的100例女性CKD患者,其中绝经前37例,绝经后63例。根据eGFR分为CKD 2期26例、CKD 3期42例、CKD 4~5期32例。另选同期健康体检女性50例作为对照组。采用化学发光微粒子免疫检测法检测患者血清中HE4的表达水平,采用脲酶-GLDH偶联速率法检测血清中尿素(Urea)水平,采用MDRD公式计算eGFR,比较绝经前后女性CKD患者中HE4的表达水平,分析HE4与Urea和eGFR的相关性。结果:CKD各期患者HE4水平均高于对照组,差异有统计学意义(P＜0.05);CKD各期患者绝经后HE4水平均高于绝经前,差异有统计学意义(P＜0.05);CKD 4~5期组HE4、Urea水平高于CKD 3期组,eGFR水平低于CKD 3期组,差异有统计学意义(P＜0.05);CKD 3期组HE4、Urea水平高于CKD 2期组,eGFR水平低于CKD 2期组,差异有统计学意义(P＜0.05),CKD 2期组HE4、Urea水平高于对照组,eGFR水平低于对照组,差异有统计学意义(P＜0.05);Pearson相关性分析表明,CKD患者HE4水平与eGFR呈负相关(r=-0.622,P＜0.05),CKD患者HE4水平与Urea呈正相关(r=0.652,P＜0.05)。结论:CKD患者绝经后HE4表达水平高于绝经前,可用于评价CKD患者的肾脏功能。Abstract: Objective: To explore theexpression level of human epididymal protein 4(HE4) in perimenopausalwomen with chronic kidney disease(CKD) and its correlation with disease stage and eGFR.Methods: A total of 100 female patients with CKD admitted to our hospital from January 2017 to June 2019 were selected, including 37 before menopause and 63 after menopause. According to the glomerular filtration rate, all patients were divided into three groups, including 26 cases of CKD stage 2, 42 cases of CKD stage 3 and 32 cases of CKD stage 4-5. Another 50 healthy female patients in our hospital during the same period were selected as the control group. Serum HE4 leve lwas measured by chemiluminescent immunoassay, serum Urea leve lwas measured by urease-gldh coupling rate, and eGFR was calculated by MDRD formula. The expression levels of HE4 in postmenopausal women with CKD were compared, and the correlation between HE4 and both Urea and eGFR was analyzed.Results: The HE4 levels of CKD patients at each stage were higher than those of the control group, and the difference was statistically significant(P＜0.05). The HE4 levels of patients at each stage of CKD after menopause were higher than those before menopause, and the difference was statistically significant(P＜0.05). HE4 and eGFR levels in the CKD 4-5 stage group were higher than those in the CKD 3 stage group and lower than those in the CKD 3 stage group, with statistically significant differences(P＜0.05). HE4 and ethylene levels in the CKD 3 stage group were higher than those in the CKD 2 stage group, and eGFR levels were lower than that in the CKD 2 stage group, with statistically significant differences(P＜0.05). HE4 and ethylene levels in the CKD stage group were higher than those in the control group, and eGFR levels were lower than that in the control group, with statistically significant differences(P＜0.05). Pearson correlation analysis showed that HE4 level in CKD patients was negatively correlated with eGFR(r=-0.622, P＜0.05), and HE4 level was positively correlated with eGFR in CKD patients(r=0.652, P＜0.05).Conclusion: The expression level of HE4 in patients with CKD after menopause was higher than that before menopause, which couldbe used to evaluate renal function in the patients with CKD.
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