Abstract: Objective: To explore the effect of different doses of gamma globulin combined with phototherapy in the treatment of children with ABO hemolysis.Methods: A total of 64 children with ABO hemolysis admitted to the Department of Blood Transfusion, People's Hospital of Longhua District, Shenzhen from February 2018 to June 2019 were selected as the research subjects, and were divided into control groups according to random sampling method(given low-dose gamma globulin combined with phototherapy) and observation group(given high-dose gamma globulin combined with phototherapy), 32 cases each, and the total clinical effective rate, adverse events rate, neonatal neurobehavioral measurement(NBNA) score, prealbumin(PA), hemoglobin(Hb), total bile acid(TBA), total bilirubin(TBiL) and time to jaundice resolution, blue light irradiation treatment time and hospital stay were observed and evaluated.Results: The total effective rate of the observation group was higher than that of the control group(χ²=5.143, P<0.05). After 72 hours of treatment, the NBNA score, PA and Hb of the observation group were higher than those of the control group, while the TBA and TBiL were lower than those of the control group(P<0.05). The jaundice resolution time, blue light irradiation treatment time, and hospital stay of the observation group were shorter than those of the control group(P<0.05). The incidence of adverse events in the observation group was the same as that in the control group(χ²=0.160, P>0.05).Conclusion: High-dose gamma globulin combined with phototherapy could better improve the NBNA score of children with ABO hemolysis, and reduce the levels of serum TBA and TBiL.