Serological detection and molecular biological analysis of CisAB blood group in children
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摘要: 目的:分析1例儿童顺式(Cis)AB血型的血清学鉴定及生物学机制。方法:采用微柱凝集法及盐水试管法进行患者血型血清学检测;采用分子生物学方法对ABO基因第1~7外显子PCR产物直接测序进行ABO血型基因分型及序列分析。结果:血清学检测结果显示患儿为ABx血型;分子生物学检测结果显示患儿样本ABO等位基因第7外显子发生467C>T、803G>C变异,鉴定结果为CisAB01/A102。结论:通过基因分型,确定该患儿血型为CisAB型;婴幼儿ABO血型抗原发育不全,容易造成血型误判,必要时应结合分子生物学检测。Abstract: Objective: To analyze the serological identification and biological mechanism of CisAB blood group in children.Methods: The blood group serological test was carried out by micro column agglutination and brine test tube method. The PCR products of exon 1-7 of ABO gene were sequenced by molecular biology method to analyze the genotypes and sequence of ABO blood group.Results: The results of serological test showed that the children were ABX. The results of molecular biology showed that the exon 7 of ABO allele in children had variations of 467 C>T and 803 G>C, and the results were CisAB01/A102.Conclusion: The blood type of the child was CisAB type by gene typing, and the development of blood-group-ABH antigens in infants was incomplete, which was easy to cause misjudgment of ABO blood type. If necessary, it should be combined with molecular biology detection.
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Key words:
- infant /
- CisAB subtype /
- gene sequencing /
- blood group serology
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