Analysis of quality indexes on leukoreduced pooled platelet concentrates with different preparation amount
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摘要: 目的 分析不同制备量的去白混合浓缩血小板的质量指标，探讨不同制备量的去白混合浓缩血小板临床使用的可行性，并为血站成分制备环节的改进提供依据。方法 将采集后1~2 d内的300 mL、400 mL全血制备成的单袋浓缩血小板按同血型随机汇集后制备成不同制备量的去白混合浓缩血小板共计60袋，分为A组(6.5~9.5 U)和B组(10.0~14.0 U)，每组各30袋。检测2组滤白前、后及储存期末的质量指标，计算并比较血小板(PLT)回收率、白细胞(WBC)清除率、红细胞(RBC)残留率。结果 2组间PLT回收率、RBC残留率及WBC清除率比较差异无统计学意义(P>0.05)；2组滤白前、后PLT、RBC计数比较差异无统计学意义(P>0.05)，WBC计数差异有统计学意义(P< 0.05)；2组滤白前、后PLT、RBC、WBC计数比较均差异无统计学意义(P>0.05)；存储期第1天和储存期末血细胞含量比较差异无统计学意义(P>0.05)；2组储存期内平均pH值连续监测结果比较，第1天差异无统计学意义(P>0.05)，第2~4天均差异有统计学意义(P< 0.05)。结论 2组的容量、PLT、RBC及pH值均符合混合浓缩血小板质量标准，可根据浓缩血小板袋数灵活制备成不同制备量的去白混合浓缩血小板，小制备量(6.5~9.5 U)的去白混合浓缩血小板应在储存期1~2 d内发往临床并尽快使用，大制备量(10.0~14.0 U)的去白混合浓缩血小板在存储期内均可用于临床。本研究PLT回收率较高，但WBC滤除率较低，提示制备环节应对离心力、过滤时间、分离手法等做进一步调整。Abstract: Objective To analyze the quality indexes of different preparation amount of leukoreduced pooled platelet concentrates, explore the feasibility of clinical use and provide the basis for the improvement of the preparation of blood components.Methods The platelets prepared from 300 mL and 400 mL whole blood within 1-2 days after collection were blended according to the same blood type and then the leukocytes were filtered to prepare 60 bags of leukoreduced pooled platelet. According to the different preparation amount, they were divided into two groups, each group 30 bags, group A(6.5-9.5 U) and group B(10.0-14.0 U). The quality indexes of the two groups were detected and compared.Results There was no significant difference in PLT recovery rate, RBC residual rate and WBC clearance rate between the two groups(P>0.05). There was no significant difference in PLT and RBC count(P>0.05) but there was significant difference in WBC count before and after filtration in group A and group B(P< 0.05). There was no significant difference in PLT, RBC and WBC counts between the two groups before and after leukocyte filtration(P>0.05). There was no significant difference in haemocytes between the first day and the last day of storage(P>0.05). There was no significant difference in pH continuous monitoring results between the two groups on the first day(P>0.05) and significant difference on the second to fourth day(P< 0.05).Conclusion The volume, PLT, RBC and pH of the two groups were all in accordance with the quality standard of pooled platelets. The preparation of different amount of leukoreduced pooled platelet can be flexibly mixed according to the number of bags of concentrated platelets. Small amount of leukoreduced pooled platelet(6.5-9.5 U) should be sent to the clinic within 1-2 days of storage period and used as soon as possible. Large amount of leukoreduced pooled platelet(10.0-14.0 U) can be used in clinic during storage period. In this study, the recovery of PLT was high, but the filtration rate of WBC was low. It is suggested that the preparation process should further adjust the centrifugal force, filtration time, separation method.
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表 1 滤白前、后血细胞计数比较
X±S 组别 例数 计量/mL PLT/(×109·L-1) WBC/(×109·L-1) RBC/(×1012·L-1) 滤前 滤后 回收率/% 滤前 滤后 回收率/% 滤前 滤后 回收率/% A组 30 238.60± 42.86 913.20± 114.16 879.10± 107.54 96.30± 1.73 0.76± 0.20 0.04± 0.03 94.27± 4.51 0.03± 0.01 0.02± 0.01 81.25± 20.75 B组 30 316.54± 41.16 991.40± 231.96 938.60± 202.21 95.03± 3.05 0.68± 0.22 0.04± 0.04 94.03± 5.12 0.02± 0.01 0.02± 0.01 82.08± 26.80 
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表 2 血细胞含量比较
X±S 项目 A组(n=30) B组(n=30) 混合浓缩血小板 滤前 滤后第1天 滤后第4天 滤前 滤后第1天 滤后第4天 PLT/(×1011个·袋-1) 2.17±0.43 2.09±0.40 2.04±0.39 3.10±0.62 2.93±0.52 2.81±0.53 ≥2.0×1010×U WBC/(×108个·袋-1) 1.84±0.64 0.24±0.16 0.23±0.15 2.14±0.71 0.32±0.20 0.29±0.19 / RBC/(×109个·袋-1) 5.73±1.87 4.62±1.96 4.47±1.88 6.44±3.29 5.13±2.83 4.86±2.65 ≤1.0×109×U
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表 3 储存期内平均pH值监测结果
组别 例数 第1天 第2天 第3天 第4天 A组 30 7.29±0.07 7.35±0.07 7.41±0.09 7.42±0.09 B组 30 7.24±0.10 7.27±0.07 7.24±0.10 7.20±0.13
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