自动细胞洗涤离心机在直接抗人球蛋白试验中的应用价值

丁梦圆; 张辉; 周群刚; 吴子豪

doi:10.13201/j.issn.1004-2806.2022.12.010

自动细胞洗涤离心机在直接抗人球蛋白试验中的应用价值

- 1.
  苏州市中心血站配型室(江苏苏州，215006)
- 2.
  苏州市中心血站体采科
- 3.
  中国科学院苏州生物医学工程技术研究所工程化研究中心
基金项目:
苏州市科教兴卫青年科技项目(No：KJXW2020057)；苏州市医学重点学科(No：SZXK202118)；苏州市姑苏卫生人才计划[No：(2022)143]

详细信息

通讯作者: 吴子豪, E-mail: wuzh@sibet.ac.cn

中图分类号: R555

收稿日期: 2022-06-09

修回日期: 2022-08-16

刊出日期: 2022-12-01

Application value of automatic cell washing centrifuge in direct anti-human globulin test

- 1.
  Matching Laboratory, Suzhou Blood Center, Suzhou, 215006, China
- 2.
  Blood Collection Department, Suzhou Blood Center
- 3.
  Research Center, Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences

More Information

Corresponding author: WU Zihao, E-mail: wuzh@sibet.ac.cn

Received Date: 09 June 2022

Revised Date: 16 August 2022

Publish Date: 01 December 2022

摘要

摘要: 目的探讨自动细胞洗涤离心机在直接抗人球蛋白试验(DAT)中的应用价值。方法随机选取20份血样，同时使用传统手工法(手工组)和自动细胞洗涤离心机(机械组)制备洗涤红细胞，比较2组的洗涤时间、对DAT检测的干扰、上清液微量蛋白和红细胞浓度。结果同时洗涤2个以上标本时，机械组所需时间短于手工组，差异有统计学意义(P＜0.05)；2种洗涤方法均可避免血浆蛋白对DAT检测的干扰；2组洗涤后上清液微量蛋白含量均较低，差异无统计学意义；但机械组洗涤后红细胞浓度较手工组显著降低，差异有统计学意义(P＜0.05)。结论应用自动细胞洗涤离心机制备洗涤红细胞，可提高实验操作效率、减少生物污染，值得在血清学实验室中推广。
- 自动细胞洗涤离心机 /
- 直接抗人球蛋白试验 /
- 洗涤红细胞
Abstract: ObjectiveTo explore the application value of automatic cell washing centrifuge in direct antiglobulin test(DAT).MethodsA total of 20 blood samples were randomly selected. Washed red blood cells were prepared by traditional manual method(manual group) and automatic cell washing centrifuge(mechanical group). The washing time, interference to DAT detection, supernatant microprotein and red blood cell concentration were compared between the two groups.ResultsWhen washing more than 2 specimens at the same time, the time required in the mechanical group was shorter than that in the manual group. The difference was statistically significant(P < 0.05). Both washing methods could avoid the interference of plasma proteins on the DAT. The microprotein content in the supernatant was lower, and the difference was not statistically significant. However, the concentration of red blood cells in the mechanical group after washing was lower than that in the manual group, and the difference was statistically significant(P < 0.05).ConclusionThe use of automatic cell washing centrifuge to prepare washed erythrocytes could improve the efficiency of experimental operation and reduce biological pollution. The centrifuge may be worthy of promotion in serology laboratories.
- automatic cell washing centrifuge /
- direct antiglobulin test /
- washing red blood cells

HTML全文

图 1 洗涤对DAT检测的影响

下载: 全尺寸图片幻灯片

表 1 2种方法洗涤后上清液微量蛋白和红细胞浓度比较 X±S

组别	例数	上清液微量蛋白含量/(g·L^-1)	红细胞浓度/%
手工组	20	0.011±0.004	0.593±0.084
机械组	20	0.008±0.004	0.231±0.062
t		1.892	20.327

下载: 导出CSV

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