Correlation between expression level of hepcidin and iron deficiency anemia in preschool children
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摘要: 目的 探讨不同铁摄入量下缺铁性贫血(iron deficiency anemia，IDA)的学龄前儿童血清中铁调素的表达并探讨其与血细胞代谢各参数的相关性。方法 研究对象共80例，按照铁摄入量分为正常铁摄入IDA组(对照组)40例，铁摄入量不足IDA组(研究组)40例。采用血细胞分析仪检测2组儿童血常规指标；用ELISA法检测血清铁调素水平，并对所有数据进行统计学分析。比较2组的一般资料、血常规指标、铁调素的表达水平。结果 对照组的铁调素水平、每日铁摄入量高于对照组(P < 0.05)。对照组铁调素与血红蛋白(Hb)、红细胞计数(RBC)、平均红细胞体积(MCV)、平均红细胞血红蛋白量(MCH)、平均红细胞血红蛋白浓度(MCHC)、红细胞比容(HCT)均呈正相关(r=0.594，0.516，0.423，0.417，0.427，0.522)，与每日铁摄入量未发现相关性(P>0.05)。研究组铁调素与Hb、RBC、MCV、MCH、MCHC、HCT均呈正相关(r=0.551，0.338，0.510，0.370，0.374，0.509)，与每日铁摄入量未发现相关性(P>0.05)。结论 铁摄入正常组的儿童铁调素高表达，血清铁调素水平在一定程度上可以反映体内缺铁的状况及程度，对指导临床诊治、观察治疗效果及预后可能具有重要价值。Abstract: Objective To investigate the expression of serum hepcidin in preschool children with iron deficiency anemia(IDA) under different iron intake and its correlation with blood cell metabolism parameters.Methods A total of 80 subjects were divided into normal iron intake IDA group(control group) 40 cases and low iron intake IDA group(study group) 40 cases according to iron intake. Blood routine indexes of the two groups were detected by blood cell analyzer. Serum hepcidin levels were determined by ELISA, and all data were statistically analyzed. The general data, blood routine indexes and the expression level of hepcidin were compared between the two groups.Results The level of hepcidin and daily iron intake in the control group were higher than those in the study group(P < 0.05). In the control group, hepcidin was positively correlated with Hb, RBC, MCV, MCH, MCHC and HCT(r=0.594, 0.516, 0.423, 0.417, 0.427, 0.522), but had no correlation with daily iron intake(P>0.05). In the control group, hepcidin was positively correlated with Hb, RBC, MCV, MCH, MCHC and HCT(r=0.551, 0.338, 0.510, 0.370, 0.374, 0.509) and there was no correlation with daily iron intake(P>0.05).Conclusion Normal iron intake group had high levels of hepcidin, The level of serum hepcidin can reflect the status and degree of iron deficiency in vivo to a certain extent, which may have important value in guiding clinical diagnosis and treatment, and observing therapeutic effect and prognosis.
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Key words:
- iron deficiency anemia /
- hepcidin /
- iron intake
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表 1 2组基线资料的比较
项目 研究组 对照组 t/Z/χ2 P 月龄/月 28.75±9.70 27.13±8.53 t=0.796 0.429 体重/kg 13.02±1.69 12.77±1.43 t=0.729 0.468 身高/cm 90.30±6.90 88.75±6.55 Z=-0.977 0.328 性别(男/女)/例 22/18 21/19 χ2=0.050 0.823 民族(维/哈)/例 7/33 5/35 χ2=0.392 0.531 MCV/fL 66.28±6.33 61.41±7.11 t=3.234 0.002 MCH/pg 19.45±2.65 17.60±3.27 t=2.782 0.007 MCHC/(g/L) 291.45±15.98 282.15±23.46 t=2.072 0.042 HCT/% 30.72±2.52 30.47±3.00 t=0.408 0.684 Hb/g 90.25±11.91 87.10±12.85 Z=-1.151 0.250 RBC/(×1012/L) 4.89±0.48 4.76±0.40 Z=-0.366 0.715 
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表 2 2组铁调素与每日铁摄入量之间的比较
X±S 项目 对照组 研究组 Z P 每日铁摄入量/mg 11.17±1.64 4.02±0.71 7.323 ＜0.001 铁调素/(ng/mL) 23.02±6.79 16.45±5.51 4.258 ＜0.001
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表 3 2组铁调素与血常规相关指标及每日铁摄入量之间的相关性
项目 对照组 研究组 r P r P Hb 0.594 < 0.001 0.551 < 0.001 RBC 0.516 0.001 0.338 0.033 MCV 0.423 0.006 0.510 0.001 MCH 0.417 0.007 0.370 0.019 MCHC 0.427 0.006 0.374 0.017 HCT 0.522 0.001 0.509 0.001 每日铁摄入量 -0.209 0.196 -0.083 0.611
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