Study on bone marrow cytomorphology inspection "two-divided" method and its application
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摘要: 目的:介绍一种新的骨髓细胞分类方法,即"二分类"法。方法:将异常类群骨髓细胞作为一类,其余所有有核细胞作为一类,进行分类计数,计算异常类群细胞的百分比。结果:使用二分类法计数10份标本的平均时间为6.56 min,使用一般方法计数的平均时间为8.61 min;使用二分类法计数10份标本,异常类群细胞百分比为(19.7±4.6)%,使用一般方法计数,异常类群细胞百分比为(19.6±5.3%);使用二分类法计数200个有核细胞的百分比为(19.7±4.6)%,计数500个有核细胞的百分比为(19.5±4.0)%。结论:二分类法完成计数的时间较短,且分类结果更精确,也更接近真实值,值得推广;当计数500个有核细胞时,分类结果更为精确。Abstract: Objective:To improve accuracy of classification results of abnormal bone marrow cells by a new method,namely "two-divided" method.Method:Abnormal bone marrow cells and other nuclear cells were divided into two groups,counted respectively and computed the percentage of abnormal bone marrow cells.Result:The average time of counting 10 samples by "two-divided" method and controversial method was 6.56 minutes and 8.61 minutes,respectively.The percentage of abnormal bone marrow cells by "two-divided" method and controversial method (both counting 200 bone marrow nuclear cells above)was (19.7±4.6)% and (19.6±5.3)%,respectively.The percentage of nuclear cells by "two-divided" method and controversial method(both counting 200 cells) was (19.7±4.6)% and (19.5±4.0)%(counting 500 cells),respectively.Conclusion:Counting cells by "two-divided" method could cost less time and gain more accurate results,particularly when counting 500 nuclear cells.
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Key words:
- bone marrow cells /
- abnormal cells /
- controversial method /
- "two-divided" method
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