Abstract: Objective:To understand the strain distribution and antimicrobial resistance of pathogens from the bile in biliary tract infection and provide the evidence for clinically anti-infective therapy.Method:Referring to National Guide to Clinical Laboratory Procedures,the bile was collected to perform bacterial culture and identification by the aseptic technique.The susceptibility testing was performed by K-B methods.And testing results were read according to the latest version of CLSI breakpoint.Result:Positive detection rate was 65.6% (231/352).The top five of isolating rate were Escherichia coli,K.peneumoniae,Enterococcus faecalis,Enterobacter cloacae and Pseudomonas aeruginasa.The susceptibility testing results indicated that except for 100% sensitivity of imipenem and meropenem against Enterobacteriaceae and of vancomycin,teicoplanin and Linezolid against gram-positive coccus,the other commonly used antibiotics were more severely resistant to pathogenic bacteria.Conclusion:The clinicians should prescribe the bile culture in time and use antibiotics according to susceptibility testing results to raise the clinical healing rate of biliary tract infection.