伤寒杆菌IVB型菌毛对THP-1细胞PKC和ERK及NF-κB活化的影响

汪付兵; 涂建成

伤寒杆菌IVB型菌毛对THP-1细胞PKC和ERK及NF-κB活化的影响

汪付兵,
涂建成^,

- 武汉大学中南医院检验科(武汉, 430071)

详细信息

通讯作者: 涂建成,E-mail:jian_1999@yahoo.com

中图分类号: R392.1

收稿日期: 2011-08-01

Effects of the Type IVB pili of Salmonella enterica serovar Typhi on the activity of PKC,ERK and NF-κB in THP-1 cells

WANG Fubing,
TU Jiancheng^,

- Department of Medical Laboratory, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China

More Information

Corresponding author: TU Jiancheng, E-mail: jian_1999@yahoo.com

Received Date: 01 August 2011

摘要

摘要: 目的:初步探讨伤寒杆菌IVB型菌毛对THP-1细胞PKC、ERK、NF-κB活化的影响。方法:将THP-1细胞分别与有IVB型菌毛的A21-6菌、缺失IVB型菌毛的pilS^-菌共同孵育,底物磷酸激酶测定法检测PKC活性变化,免疫印迹法检测ERK磷酸化水平,荧光素酶报道基因分析法检测NF-κB激活表达。结果:THP-1细胞经有IVB型菌毛的A21-6菌刺激后,PKC活性变化、ERK磷酸化水平和NF-κB激活表达均显著高于相应的处理组;PKC、ERK、NF-κB活化水平分别于刺激10、30、60 min后即明显升高;并分别于30、60、120 min左右达到峰值。结论:伤寒杆菌IVB型菌毛显著增强了THP-1细胞PKC、ERK、NF-κB活化水平,IVB型菌毛在伤寒杆菌诱导单核/巨噬细胞PKC、ERK、NF-κB活化过程中是一种重要的强刺激因素。
- 伤寒杆菌IVB型菌毛 /
- THP-1细胞 /
- PKC /
- ERK /
- NF-κB
Abstract: Objective:To investigate the effects of the Type IVB pili of Salmonella enterica serovar Typhi on the activity of PKC, ERK and NF-κB in THP-1 cells.Method:THP-1 cells were incubated with the Salmonella Typhi strain A21-6 or Salmonella Typhi strain pilS^-,the activity of PKC, ERK and NF-κB were measured with substrate phosphorolysis kinase assay,Western blotting and luciferase reporter gene assay,separately . Result: The Type IVB pili of Salmonella entrica serovar Typhi strain A21-6 was significantly effected on the activity of PKC, ERK and NF-κB;the activity of PKC, ERK and NF-κB were increased 10min、30min、60min after being stimulated with the Salmonella Typhi A21-6,and reached the peak value at 30min, 60min and 120min,respectively. Conclusion: The type IVB pili of Salmonella Typhi may play an important role in the activity of PKC, ERK and NF-κB induced by Salmonella Typhi,which could significantly increase the activity of PKC, ERK and NF-κB in THP-1 cells.
- type IVB pili of Salmonella enterica serovar Typhi /
- THP-1 cell /
- PKC /
- ERK /
- NF-κB

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