微柱凝胶法对南京地区孕妇人群不规则抗体的检测分析

许进明; 周小玉; 朱永亮; 华岚; 张敏

微柱凝胶法对南京地区孕妇人群不规则抗体的检测分析

- 南京医科大学第一附属医院输血科(南京, 210029)

详细信息

通讯作者: 周小玉,E-mail:deerzxy@163.com

中图分类号: R457.1

收稿日期: 2011-10-01

Detection of irregular antibodies by micro-gel test among pregnant women in Nanjing province

- Department of Blood Transfusion, the First Hospital of Nanjing Medical Uiversity, Najing, 210029, China

More Information

Corresponding author: ZHOU Xiaoyu, E-mail: deerzxy@163.com

Received Date: 01 October 2011

摘要

摘要: 目的:探讨本地区孕妇人群不规则抗体的阳性率及分布。方法:使用微柱凝胶法对南京地区3 256例孕妇的血清进行不规则抗体的检测,对阳性者进行抗体特异性、免疫球蛋白类型、效价及37℃反应性的检测。结果:3 256例孕妇中检测出特异性不规则抗体16例,阳性率为0.49%。微柱凝胶法对抗体特异性鉴定为抗-Lea 1例,抗-M 3例,抗-E 5例,抗-D 4例,抗-Ec 2例,抗-c 1例。结论:特异性不规则抗体在本地区的分布较多的为抗-E、抗-M和抗-D,检测孕妇血型不规则抗体对早期诊断非ABO-HDN、为孕妇准备相合的血源以避免紧急输血时的配血困难都具有十分重要的临床意义。
- 不规则抗体 /
- 孕妇 /
- 血型 /
- HDN
Abstract: Objective:To analyze the positive rate and distribution of irregular antibodies among pregnant women in Nanjing Province. Method:The irregular antibodies in maternal serum of 3256 pregnant women were detected using micro-gel test. The specificity of the antibody, type of immunoglobulin, titer and antibody reaction at 37℃ was determined for those pregnant women who tested positive for irregular erythrocyte antibody. Result:Sixteen out of the 3256 cases were positive for irregular antibodies, with positive rate of 0.49%. The specificity of 20 cases of irregular antibodies was as follows: 1 case of anti-Le (a), 3 cases of anti-M, 5 cases of anti-E, 4 cases of anti-D, 4 cases of anti-Ec and 1 case of anti-c.Conclusion:Anti-C,anti-E and anti-D occurred more frequently in this area. The detection of irregular RBC antibody might be of important clinical significance in early diagnosis of non-ABO-HDN and preparing matching blood in a timely manner for pregnant women to prevent transfusion reactions caused by the blood group incompatibility.
- irregular antibodies /
- pregnant women /
- blood type /
- Haemolytic disease of the newborn（HDN）

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参考文献(8)

[1]	曹奎杰,胡丽华,刘峰.不规则抗体检查在临床输血中的应用[J].临床血液学杂志,2002,15(4):163-164.
[2]	吴敏华容伯芬.卡式微柱凝胶法在抗体筛查中的应用[J].中国医学研究与临床,2004,2(16):75-76.
[3]	李勇,杨贵贞.人类红细胞血型血清学实用理论与实验技术[M].北京:中国科学技术出版社,1999:227-227.
[4]	王培华.输血技术学[M]. 2版.北京:人民卫生出版社,2003:222-225.
[5]	WINTERS J L, PINEDA A A, GORDEN L D et al. RBC alloantibody specificity and antigen potency in Olmsted County, Minnesota[J]. Transfusion,2001,41:1413-1420.
[6]	董永,李延年,孙波,等.微柱凝集法筛检不规则抗体的临床应用[J].中国输血杂志,2003,16(5):326-327.
[7]	吴远军,刘彦慧,刘兴玲,等.汉族患者(30800例)及孕妇(4200例)红细胞血型不规则抗体分布的调查[J].第四至医大学学报,2007,28(10):922-925.
[8]	吴远军,周皓云,刘兴玲,等.孕妇血型不规则抗体检测的临床意义[J].中国实验诊断学,2005,9(4):518-520.