Distribution of HLA-B27 subtypes in Han Chinese population of southwest Shandong and their association with ankylosing spondylitis
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摘要: 目的:调查鲁西南汉族人群中正常人和强直性脊柱炎(AS)患者的HLA-B*27基因亚型的频率分布状况,并分析HLA-B*27亚型与AS的相关性。方法:对826名正常人(对照组)、76例AS患者(AS组)采用特异性引物聚合酶链反应技术(PCR-SSP)进行HLA-B*27基因及亚型检测。结果:在正常组和AS 组中,共检出了11种HLA-B*27亚型,2组均以 B*2704和 B*2705 亚型为优势亚型,2亚型分别为38.8%和41.8%。2组间的 B*2704和 B*2705亚型的构成比差异无统计学意义。结论:HLA-B*2704和HLA-B*2705是鲁西南地区汉族人群AS的易感基因,与鲁西南地区汉族人群AS的发病呈强相关。Abstract: Objective:To investigate the frequency of HLA-B*27 gene and the distribution of its alleles in healthy individuals and patients with ankylosing spondylitis(AS)in Chinese Han nationality of Shandong region,and to analyze the association between the heterogeneity of HLA-B27 and AS.Method:HLA-B*27 alleles in 826 cases of healthy individuals and 76 cases of AS patients were typed by PCR amplification with sequence-specific primers.Result:Eleven HLA-B*27 genotypes were determined in healthy individuals and AS patients. HLA-B*2704 and HLA-B*2705 were the two high frequency genotypes,with a genotype frequency of 38.8％ and 41.8％.HLA-B*2702 was detected only in one case in the AS patients,and HLA-B*2701, HLA-B*2713 and HLA-B*2721 were detected only in one case in the controls respectively.Conclusion:HLA-B*2704 and HLA-B*2705 were the primary genes susceptive to AS in Chinese Han nationality of southwestern Shandong, which were strongly associated with AS.
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Key words:
- ankylosing spondylitis /
- HLA-B27 gene /
- HLA-B27 genotype
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