激活素A对LX-2分泌细胞外基质的影响

汪涌; 方胜英; 朱励; 祝剑方; 曾令兰

激活素A对LX-2分泌细胞外基质的影响

- 1 华中科技大学同济医学院附属协和医院感染科实验室(武汉, 430022);
- 2 华中科技大学同济医学院附属协和医院中心实验室

详细信息

通讯作者: 曾令兰,E-mail:xhyycrk@163.com

中图分类号: R329.2

收稿日期: 2012-06-26

Effects of activin A on extracellular matrix secretion of LX-2

- 1. The Department of Infections Diseases Laboratory, Affiliated Union Hospital of Tongji Medical College, Huazhong University of Seience and Technology, Wuhan, 430022, China;
- 2. Center Laboratory, Affiliated Union Hospital of Tongji Medical College, Huazhong University of Seience and Technology

More Information

Corresponding author: CENG Linglan, E-mail: xhyycrk@163.com

Received Date: 26 June 2012

摘要

摘要: 目的:了解激活素A(ACTA)对体外培养的人肝星状细胞(HSC)系LX-2细胞分泌细胞外基质(ECM)的影响。方法:将培养的LX-2细胞分成5组,除正常对照组外,其余4组分别加入浓度为10、20、100、200 μg/L ACTA,作用24、48和72 h后取培养上清,用化学免疫发光法检测Ⅲ型前胶原N端肽(PⅢNP)、Ⅳ型胶原(Ⅳ-COL)、透明质酸(HA)及层粘连蛋白(LN)的含量。结果:ACTA刺激后的PⅢNP、Ⅳ-COL和HA表达水平均呈上升趋势,表现出与时间和剂量较强的依赖关系(成正比);而LH仅在后期(72 h)将ACTA上调到 200 μg/L时,与正常对照组比才出现统计性差异。结论:ACTA能成功诱导HSC分泌ECM;PⅢNP等4项指标均能反映ACTA参与了肝纤维化的发生发展过程以及肝纤维化病理程度;如何阻断ACTA途径,为今后预防和治疗肝纤维化提供了新思路。
- 激活素A /
- 肝星状细胞 /
- 细胞外基质 /
- 肝纤维化
Abstract: Objective:To learn the effects of activin A on the secretion of extracellular matrix(ECM) in vitro cultured LX-2 cell.Method:Cultured LX-2 cells were divided into five groups. In addition to the normal control group,the rest four groups were added different concentrations of activin A 10,20,100 and 200 μg/L, respectively. The contents of amino-terminal peptide of procollagen type Ⅲ(PⅢNP),collagen type Ⅳ(Ⅳ-COL),hyaluronic acid(HA)and laminin(LN)in culture supernatants were detected by chemiluminescence after 24, 48 and 72 h, respectively.Result:The secretion of PⅢNP,Ⅳ-COL and HA in HSC were enhanced by activin A according to its concentration(10-200 μg/L)in a time dose-dependent manner. Activin A had no effect on LN at 10-100 μg/L(P>0.05),while significant difference was presented after 72 h in the 200μg/L activin A intervention group than that in the normal control group(P<0.01).Conclusion:ACTA could induce HSC secrete ECM successfully. PⅢNP et al four indexes could reflect that ACTA might participate the occurrence and developing process of liver fibrosis and pathological degree. How to block ACTA way in the prevention and treatment of liver fibrosis provides us some new ideas.
- activin A /
- hepatic stellate cell /
- extracellular matrix /
- hepatic fibrosis

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