Detection analysis of drug resistant mutations and genotyping of hepatitis B virus
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摘要: 目的:检测武汉地区乙型肝炎病毒的基因型与耐药突变基因,为临床治疗乙型肝炎患者提供实验室依据。方法:采用PCR-反向斑点杂交法对308例乙肝患者进行乙肝病毒基因型分析,同时检测拉米夫定耐药突变基因和阿德福韦酯耐药突变基因。结果:308例乙肝患者中检出B基因型173例,C基因型78例,B/C混合基因型6例,未检出51例。123例(39.94%)患者的HBV出现拉米夫定耐药,涉及3个位点4个突变型;34例(11.03%)出现阿德福韦酯耐药突变,涉及2个位点2个突变型。结论:武汉地区乙肝病毒存在较高的耐药基因突变率,对乙肝患者进行抗病毒治疗前和治疗过程中,应检测耐药基因是否出现突变。Abstract: Objective:To investigate the distributions of genotyping and drug resistance mutations of hepatitis B virus in Wuhan. Method:Serum samples were collected from 308 patients with HBV in Wuhan and all the samples were detected by reverse dot blot about the genotyping and drug resistance mutations. Result:Of the 308 patients, 173 samples were genotype B and 73 samples were genotype C. Only 6 samples were both genotype B and C. 51 samples were undectected. Lamivudine resistant mutations which involving 4 mutations of 3 loci were detected in 123 (39.94%) of 308 patients with HBV, while adefovir resistant mutations detected in 34 (11.03%) which involving 2 mutations of 2 loci. Conclusion:There was high rate of hepatitis B virus resistance mutations in Wuhan, suggesting that the drug resistance mutations of hepatitis B virus in patients should be detected before and after anti-virus treatment.
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Key words:
- hepatitis B virus /
- drug resistant gene /
- genotype /
- mutation
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[1] 中华医学会肝病学分会.慢性乙型肝炎防治指南(2015)[J].中华肝脏病杂志,2015,23(12):888-905.
[2] Chen X,Gao J,Ji Z,et al. A description of the hepatitis B virus genomic background in a high-prevalence area in China[J]. Virol J, 2014,11:101.
[3] 欧阳耀灵,杨忠民,刘顺等.HBV基因分型和耐药突变基因检测[J].中国卫生检验杂志,2015,25(13):2176-2178.
[4] 李雅娟,庄辉,李杰,等.乙型肝炎病毒感染者病毒基因型和亚型分布及其临床意义[J].中华肝脏病杂志,2005,13(10):724-729.
[5] 王晓,刘妍,思兰兰,等.阿德福韦酯治疗失败的慢性乙型肝炎患者HBV反转录酶区新变异rtN236V的表型耐药特点分析[J].解放军医学杂志,2013,38(4):256-259.
[6] Tacke F, Kroy DC.Treatment for hepatitis B in patients with drug resistance[J].Ann Transl Med,2016,4:334.
[7] Lim YS.Management of antiviral resistance in chronic hepatitis B[J].Gut Liver,2017,11:189-195.
[8] European Association For The Study Of The Liver. EASL clinical practice guidelines:Management of chronichepatitis B virus infection[J]. J Hepatol, 2012,57:167-85.
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