应用微柱凝胶技术评估并优化IgG抗A/B吸收试验

刘峰; 李归宁; 李敏芳; 张敏; 胡丽华

应用微柱凝胶技术评估并优化IgG抗A/B吸收试验

- 华中科技大学同济医学院附属协和医院输血科(武汉, 430022)
基金项目:
卫生部卫生行业科研专项项目(No:200902008-07)

详细信息

通讯作者: 胡丽华,E-mail:xhhlh@126.com

中图分类号: R446.6

收稿日期: 2012-04-29

Application microcolumn gel technique to assess and optimize the adsorption test of IgG anti-A/B

- Department of Blood Transfusion, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

More Information

Corresponding author: HU Lihua, E-mail: xhhlh@126.com

Received Date: 29 April 2012

摘要

摘要: 目的:探讨适用于以微柱凝胶技术为基础的IgG抗A/B吸收试验。方法:选取30例IgG抗A及抗B起始效价从64~1 024的血清样本,与A型或B型红细胞于37℃进行吸收试验,于5 min、10 min、30 min、1 h、2 h、4 h吸收时间后分离并测定吸收后血清的效价。结果:①IgG抗A和抗B均存在吸收效果伴随吸收时间变化的趋势,起始效价在128~1 024基本上于2 h后进入平台期。②IgG抗A和抗B 起始效价为128与256,512与1 024的时间变化趋势均差异有统计学意义(均P<0.05);IgG抗B起始效价为256与512、1 024之间的时间变化趋势均差异有统计学意义(均P<0.05)。③相同吸收时相的IgG抗A 与抗B吸收效果之间差异无统计学意义(P＞0.05)。结论:在依赖微柱凝胶技术条件下,采取37℃ 2 h吸收1次后再结合1次1~2 h就能对起始效价小于1 024的IgG抗A和抗B能使其充分吸收。
- 微柱凝胶技术 /
- 吸收试验 /
- IgG /
- 抗A/B /
- 效价
Abstract: Objective:To investigate the optimal protocol of IgG anti-A/B adsorption test based micro column gel technique.Method:30 random cases of serum samples with IgG anti-A or anti-B were collected and assimilated by the corresponding A or B cells,then the isolated serum post adsorption test contained the reaction time of 5 min,10 min,30 min,1 h,2 h,4 h,respectively.Result:①The alterative tendency of adsorption effects varied in the different adsorption time,and the platform stages of adsorption effects in the initial titration from 128 to 1 024 occurred after about 2 hours.②The tendency of adsorption effects along with time in IgG anti-A and anti-B with the initial titration of 128 were significant different from those initial titration of 256,512 and 1 024(P<0.05),the same was true of IgG anti-B with the initial titration between 256 and 512 or 1 024(P<0.05).③In the same adsorption time,the tendency of adsorption effects between IgG anti-A and IgG anti-B were no obvious difference(P＞0.05).Conclusion:Combined an adsorption test with 2 hours in 37℃ and another adsorption test with 1 to 2 hour,the largest efficacy in adsorption test of IgG anti-A/B whose initial titration is beneath 1 024 can be obtained.
- microcolumn gel technique /
- adsorption test /
- IgG /
- anti-A/B /
- titration

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