2种试剂红细胞用于血型反定型检测的效果比较

赵磊; 储倩; 许婷婷; 张红; 凃历波; 王先广

2种试剂红细胞用于血型反定型检测的效果比较

- 1 武汉血液中心检验科(武汉, 430030);
- 2 大连医科大学检验医学院

详细信息

通讯作者: 赵磊,E-mail:myrelay@126.com

中图分类号: R446.6

收稿日期: 2012-05-21

Comparative analysis of effects of different reagent red cells in reverse ABO grouping

- 1. Department of Laboratory, Wuhan Blood Center, Wuhan, 430030, China;
- 2. College of Laboratory Diagnostic Medicine, Dalian Medical University

More Information

Corresponding author: ZHAO Lei, E-mail: myrelay@126.com

Received Date: 21 May 2012

摘要

摘要: 目的:比较商品试剂红细胞和自制试剂红细胞在血型反定型检测中的效果。方法:应用商品试剂红细胞和自制试剂红细胞在梯形微板中进行反定型检测,比较两种试剂红细胞的一次性判读结果的准确率、亚型和不规则抗体的检出率、手工改动现象、灵敏度及抗干扰能力。结果:商品试剂红细胞同自制试剂红细胞相比,前者的一次性判读准确率较高,手工改动现象较少,灵敏度较稳定,在溶血中抗干扰能力较强,而后者的不规则抗体的检出率较高,脂血的抗干扰能力两者接近。结论:商品试剂红细胞用于血型反定型检测的效果略优于自制试剂红细胞,不同的实验室应结合实际情况进行选择。
- 试剂红细胞 /
- 血型反定型 /
- 梯形微板法
Abstract: Objective:To compare the effects of home-made reagent red cells and commercial reagent red cells in reverse ABO grouping.Method:We perform parallel reverse ABO grouping tests on samples from blood donors with home-made reagent red cells and commercial reagent red cells chosen from market.The accuracy test,detection rate of subtype and irregular antibody,manual change phenomenon,sensitivity test,lipaemia interference test and haemolysis interference test were carried out.The performances between two different reagent red cells were compared.Result:Compared to home-made reagent red cells,commercial reagent red cells had higher accuracy,steadier sensitivity,lesser manual change and stronger anti-interference ability in haemolysis environment.Home-made reagent red cells had higher detection rate of subtype and irregular antibody.The correct interpretation rates of them were identical in lipaemia interference test.Conclusion:Conclusively,commercial reagent red cells might be superior to home-made reagent red cells under many circumstances,and home-made reagent red cells might have the advantages of flexibility and lower cost.Laboratories could choose appropriate reagent red cells according to actual situation and fully utilize them.
- reagent red cell /
- reverse ABO grouping /
- trapezoidal microplate method

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