Abstract: Objective:To understand drug-resistance of clinical isolates of gram-negative bacilli(GNB)in county hospital from 2010 to 2011 and provide the referrence for clinical reasonable use of antibiotics.Method:Pathogenic bacteria were cultured according to the National Guide to Clinical Laboratory Procedures. API system(bioMérieux,France)was used to identify the strain. Drug susceptibility testing was performed by K-B methods recommended by CLSI. And results were assessed. Result:732 GNB were collected in our Hospital from 2010 to 2011.The detection rates of ESBLs producing E.coli and Klebsiella were 65.6% and 37.9%,respectively. Resistance of imipenem against GNB was the lower and all less than 7%.Resistance rates of piperacillin/tazobactam and amikacin against Enterobacter were also lower,being 22.1%.Resistance rates of ciprofloxacin against Shigella,Citrobacter and Serratia were less than 25%.Resistance rates of most antibiotics against Proteus were below 20%. Resistance rates of ceftazidime and ciprofloxacin against Pseudomonas aeruginosa were 14%,while gram-negative bacilli performed higher resistance to most other antibiotics. Conclusion:Resistance of commonly used antibiotics against GNB was severe in our hospital. We should strengthen management of antibiotics use to restrain the increasing tendency of bacterial resistance.