不同反应条件的半乳甘露聚糖检测的研究

何静; 熊永芬; 周强; 李鹏程; 王顺

doi:10.13201/j.issn.1004-2806-b.2018.08.014

不同反应条件的半乳甘露聚糖检测的研究

- 武汉市中西医结合医院/武汉市第一医院输血科(武汉, 430022)
基金项目:
国家自然科学基金项目(No:81301507)

详细信息

通讯作者: 王顺,E-mail:wang_shun6688@sina.com

中图分类号: R446.5

收稿日期: 2017-11-30

Analyses of different methods of GM test

- Deptartment of Transfusion Medicine, Wuhan Integrated Traditional Chinese and Western Medicine Hospital/Wuhan NO. 1 Hospital, Wuhan, 430022, China

More Information

Corresponding author: WANG Shun, E-mail: wang_shun6688@sina.com

Received Date: 30 November 2017

摘要

摘要: 目的:半乳甘露聚糖(GM)试验是一种诊断侵袭性曲霉菌感染(IA)的早期抗原指标,本实验旨在探索孵育方式与显色时间对GM试验结果的影响。方法:采集武汉市第一医院137例住院患者的血清或肺泡灌洗液样本进行实验,按不同的孵育方式和显色时间分别将实验进行分组,计算不同组的阳性率,再用SPSS 13.0软件进行统计学分析。结果:取待测样本与标准血清的吸收度的比值(I值) ≥ 0.5为阳性,不同实验组的阳性率均差异有统计学意义(P<0.05)。结论:孵育方式和显色时间对GM试验的结果有显著影响。
- 侵袭性真菌感染 /
- 侵袭性曲霉菌感染 /
- GM试验 /
- 孵育方式 /
- 显色时间
Abstract: Objective:Galactomannan(GM)test is an early antigen index for diagnosis of invasive aspergillus infection(IA).The aim of this experiment is to investigate the effects of different incubation conditions and coloration time on GM test.Method:A total of 137 inpatients with serum or alveolar lavage fluid were enrolled in our hospital as the test sample.The positive rate of different groups was calculated by grouping the experiment according to different incubation conditions and different coloration time.Statistical analysis was taken using SPS S13.0 software.Result:The ratio of the absorbance of the test sample to the standard serum(I value)was greater than 0.5,and both of the differences of incubation conditions and coloration time had statistical significant(P<0.05).Conclusion:Incubation conditions and coloration time may both have significant effect on the results of the GM test.
- invasive fungal infections /
- invasive aspergillosis /
- galactomannan test /
- incubation conditions /
- coloration time

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参考文献(5)

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