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摘要: 目的:提高全自动血型仪献血者初筛血型检测结果的准确性,分析在使用过程中遇到的判读无效结果的原因,制定应对策略。方法:收集伽利略血型仪对2016-08-2017-07献血者标本210 055份进行ABO、Rh(D)血型检测,对血型仪判读无效的结果进行分析。通过试管法复查或送至中心血型研究室进行最终血型确认。结果:一次判读正确的标本208 785份(99.4%),判读无效的标本1 270份(0.6%),经血型研究室确认78份ABO正反定型不符的标本中包括冷抗体5份(6.4%)、特异性抗体19份(24.4%)、抗原减弱20份(25.6%)、抗体减弱34份(43.6%);其余1 192份无法判读结果的标本中根据ABO、Rh检测过程中出现的情况分为灰区127份(10.7%)、96份(8.1%);未加上样本376份(31.5%)、376份(31.5%);阴性质控结果异常325份(27.3%)、325份(27.3%);脂血90份(7.5%)、0份(0);污染149份(12.5%)、5份(0.4%);未加上试剂8份(0.7%)、16份(1.3%)。结论:全自动血型仪操作简便,检测结果能长时间保存,易于追溯。对于判读无效的结果结合手工试管法可提供高效准确的检验结果,确保临床用血安全。Abstract: Objective: To investigate the accuracy of full-automated blood typing instrument in the identification of blood types,and analyze the causes of invalid results in our tests and then formulate appropriate strategies. Method:From August 2016 to July 2017,210 055 blood samples were submitted to ABO and Rh(D)identification by GALILEO NEO full-automatic blood typing instrument.The samples of invalid results were reexamined by the tube method or were sent to blood typing laboratory for confirmation and the reasons were analyzed. Result:208 785(99.4%)were correctly identified,and 1 270(0.6%)were invalid.There were 78 specimens that were confirmed by the blood type research room as inconsistent with positive and negative ABO stereotypes,and among them,5 cases(6.4%)of cold antibodies,19 cases(24.4%)of specific antibodies,20 cases(25.6%)of weakened antigens,and 34 cases(43.6%)of weakened antibodies.According to ABO and RH tests,the remaining 1,192 specimens that could not be interpreted were divided into two groups.The causes of first group were 127 cases(10.7%)grey areas,376 cases(31.5%)not added to the sample,325 cases(27.3%)Abnormal results of negative quality control,90 cases(7.5%)Lipid blood,149 cases of pollution(12.5%),8 cases(0.7%)reagents not added.The corresponding reasons for the second groups were 96 cases(8.1%),376 cases(31.5%),325 cases(27.3%),0 cases(0),5 cases(0.4%),16 cases(1.3%).Conclusion:The full-automatic blood typing instrument was convenient operation,the test results can be preserved for a long time,easy to trace.The results of invalid interpretation combined with manual test tube method can provide efficient and accurate test results to ensure the safety of clinical blood use.
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Key words:
- full-automated blood typing instrument /
- invalid results /
- blood safety
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