Comparison of automatic polybrene microplate method and gel microcolumn method in detection of red blood cell antibodies
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摘要: 目的:比较全自动凝聚胺微板法和微柱凝胶法检测红细胞抗体的优劣,为实验室选择试验方法提供依据。方法:用2种方法对64例阳性抗体和64例阴性标本进行平行检测。结果:凝聚胺微板法检测出62例,2例抗-Lea漏检;微柱凝胶法检测出49例,13例IgM抗体及2例抗-E抗体漏检。结论:全自动凝聚胺微板法对抗体的检出率高。Abstract: Objective:To compare the automatic polybrene microplate method and gel microcolumn method in detection of red blood cell antibodies.Method:We parallelly detected 64 positive samples and 64 negative samples with two methods.Result:Sixty-two positive samples were found and 2 samples of anti-Lea failed to be found by Polybrene microplate method.Forty-nine positive samples were found,13 samples of IgM antibody and 2 samples of anti-E failed to be found by gel microcolumn method.Conclusion:Automatic Polybrene microplate method has higher detection rate of antibodies.
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Key words:
- polybrene microplate method /
- gel microcolum method /
- irregular antibodies
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